Course: Seminar of Separation Methods

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Course title Seminar of Separation Methods
Course code KALCH/C052
Organizational form of instruction Seminar
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 1
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Fischer Jan, doc. Ing. CSc.
  • Česla Petr, doc. Ing. Ph.D.
Course content
Qualitative analysis - retention. Dead retention time and volume, mobile phase flow, pressure drop correction factor. Retention characteristics, absolute and relative correlations. Column characteristics - efficiency, number of theoretical plates, height equivalent of theoretical plate, resolution, separation number, required number of plates. Processing of TLC chromatograms. Processing of electropherograms and outputs from other electromigration techniques. Quantitative analysis in chromatography, electrophoresis, detector response, limit of detection. Working techniques of quantitative analysis.

Learning activities and teaching methods
Work with text (with textbook, with book), Methods of individual activities
Learning outcomes
The aim of the course is to practice basic knowledge in the field of chromatographic and electromigration separation analytical techniques.
Couarse graduate is able to interpret chromatograms and electropherograms and process chromatographic data in terms of quantitative and qualitative analysis and evaluation of separation techniques.
Prerequisites
Knowledge within the range of bachelor studies at FCHT is required.

Assessment methods and criteria
Written examination

The assignment is granted upon a successful passing of a written paper - minimum is 50 %.
Recommended literature
  • Churáček J. a kol. Analytická separace látek, 1990, SNTL Praha..
  • Churáček J. a kol. Nové trendy v teorii a instrumentaci vybraných analytických metod. Praha, 1993.
  • Krejčí M., Pajurek J., Komers R. a kol. Výpočty a veličiny v sorpční kolonové chromatografii. Praha, 1990.
  • Smolková E., Feltl L. Analýza látek v plynném stavu. Praha, 1991.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Chemical Technology Study plan (Version): Evaluation and Analysis of Foodstuffs (2013) Category: Food industry and food industry chemistry 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Chemical Technology Study plan (Version): Analytical Chemistry (2013) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Chemical Technology Study plan (Version): Evaluation and Analysis of Foodstuffs (2016) Category: Food industry and food industry chemistry 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Chemical Technology Study plan (Version): Analytical Chemistry (2015) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Chemical Technology Study plan (Version): Evaluation and Analysis of Foodstuffs (2015) Category: Food industry and food industry chemistry 1 Recommended year of study:1, Recommended semester: Summer
Faculty: Faculty of Chemical Technology Study plan (Version): Analytical Chemistry (2016) Category: Chemistry courses 1 Recommended year of study:1, Recommended semester: Summer