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Lecturer(s)
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Bajer Tomáš, doc. Ing. Ph.D.
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Bajerová Petra, prof. Ing. Ph.D.
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Husáková Lenka, doc. Ing. Ph.D.
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Mikysek Tomáš, doc. Ing. Ph.D.
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Course content
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Basic concepts of analytical chemistry Tasks of analytical chemistry, proof, determination. Expression of active substance content in solutions. Sample collection and preparation for analysis. Concentration, basic extraction techniques. Qualitative analysis of inorganic substances - chemical reactions, evidence of cations and anions. Chemical equilibria in solutions. Proportional analysis, requirements for the course of the reaction. Measuring solution, titre, basic substances, equivalence point, titration curve. Direct, indirect and back titration. Protolytic, complexing, precipitation and oxidation-reduction reactions. Use in analytical chemistry - volumetric analysis, applications. Basic procedures in the analysis of organic substances. Qualitative and quantitative elemental analysis. Determination of some functional groups. Electroanalytical methods. Potentiometry and potentiometric titrations. Principles of techniques at measurement under passage of current. Heyer polarography, voltammetry. Sample preparation for voltammetry and polarography and their use in practice. Amperometry, Clark electrode, biosensors. Coulometry, coulometric titrations with electrolytic generation of titrants. Examples of determinations. Conductometry, conductivity in solution, conductometric titrations, titration curves. Spectral and optical methods. Introduction to atomic spectrometry, classification of methods. Atomic absorption and optical emission spectrometry - principles, analytical applications. X-ray fluorescence spectrometry and mass spectrometry with ionisation in inductively coupled plasma - possibilities of qualitative and quantitative analysis, practical applications. Molecular absorption spectrometry (UV/VIS, IR/Raman) - principles, radiation characteristics, instrumentation, spectra generation, applications, interpretation of measured data. Polarimetry, nephelometry, turbidimetry. Separation methods. Basic principles of separation. Gas, liquid, paper and thin layer chromatography. Electromigration separation methods. Coupling of mass spectrometry with separation techniques. Evaluation of the results of chromatographic measurements. Processing of analytical results Calibration methods. Validation parameters. Evaluation of the analysis.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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The aim of the course is to provide students with a solid and sufficiently broad theoretical and methodological basis for the rational provision of chemical analysis of inorganic and organic substances. The focus of the course is to explain the theoretical basis of chemical methods of analysis. In practical exercises, emphasis is placed on the students' independent approach to solving analytical problems.
By completing the course C197 "Analytical Chemistry for Industrial Practice" the student will gain an overview of working in an analytical laboratory with basic equipment.
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Prerequisites
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High school knowledge of analytical chemistry.
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Assessment methods and criteria
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Oral examination, Written examination, Student performance assessment
Written part of the exam - 40% of the exam Oral part of the exam - 60% of the exam
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Recommended literature
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Barek J., Opekar F. a Štulík K. Elektroanalytická chemie, Karolinum Praha 2005.
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Bartoš, Martin. Analytická chemie. Pardubice: Univerzita Pardubice, 2018. ISBN 978-80-7560-188-9.
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Eisner, Aleš. Laboratorní cvičení z Analytické chemie. Pardubice: Univerzita Pardubice, 2020. ISBN 978-80-7560-326-5.
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Klouda P. Moderní analytické metody, Klouda 2003.
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Labuda J a kol. Analytická chémia, 2014, Slovenská technická univerzita v Bratislavě. 2014.
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Opekar F. a kol. Základní analytická chemie. Univerzita Karlova v Praze: Karolinum, 2003.
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Štulík K. a kol. Analytické separační metody, Karolinum Praha 2004.
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Vláčil F.a kol. Příklady z chem. a inst. analýzy, 1983, SNTL Praha; 1991, Informatorium Praha..
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