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Lecturer(s)
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Ventura Karel, prof. Ing. CSc.
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Bajerová Petra, prof. Ing. Ph.D.
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Course content
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Introduction to analytical toxicology (fundamental terms, relations between dose and toxicity) Review of toxicologically active compounds, natural toxins, phatmaceutical toxicology Mechanisms of toxic effects and biotransformation (exposures of toxic effects, transport of toxicant in organism, excretion of xenobiotics) Experimental toxicology (evaluation of toxic properties of chemicals, manipulation with laboratory animals, toxicity determination), biological exposure tests Systematic toxicological analysis, screening and confirmation methods (screening tests based on coloured chemical reactions, chromatographic and immunochemical screening tests), Sample pre-treatment for individual goals of toxicological examination Modern electrochemical methods in biologically active organic compounds analysis, biological exposure monitoring, HPLC equipped with electrochemical detection Chromatographic methods in toxicology Mass spectrometry methods in toxicology Hormones, steroids and their analysis Doping, work in the control laboratory, purity control of food supplements Legislation and manipulation with toxins and other harmful substances, controlled compounds and their precursors Toximania (toxic effects abuse, factors affecting the toximania creation, types of drug dependency characters) Forensic and clinical toxicology, occupational medicine, ecotoxicology, phytotoxicology, military toxicology, industrial toxicology
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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The aim of this course is to enrich the basic knowledge of students in the field of fundamental toxicology in term of toxicological examination of various biological samples using modern analytical approaches in combination with the knowledge about the destiny of xenobiotics in organism.
Students after graduation of the course are introduced with the possibilities of instrumental methods application for the analysis of biological samples.
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Prerequisites
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High school knowledge of analytical chemistry
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Assessment methods and criteria
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Oral examination, Written examination
Written test - 70 % of grade Oral examination ? 30 %
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Recommended literature
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Churáček J., Jeník J., Chýlková J., Horna A. Analytická toxikologie. Pardubice, 1984.
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Prokeš, J. Základy toxikologie 1, 2. Praha, 1997.
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Ventura K. Příprava vzorku ve stopové analýze organických látek. Extrakce kapalinou, plynem, sorbentem, superkritická fluidní extrakce a chromatografie. Pardubice, 1995.
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Vopršálová, M. Základy toxikologie. Praha, 1996.
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