1. week: Introduction to mass spectrometry (history, basic terms in MS, basic principles of MS, basic parts of MS, detectors, vacuum technology, ion optics) 2. week: Ionization techniques - part 1 (electron ionization, chemical ionization, ionization techniques working at atmospheric pressure (ESI, APCI, APPI)) 3. week: Ionization techniques - part 2 (MALDI, ambient ionization techniques - DESI, DART) 4. week: Mass analyzers - part 1 (basic definition, magnetic sector analyzer, Q, IT, LIT) 5. week: Mass analyzers - part 2 (TOF, FT-ICR, Orbitrap, tandem mass spectrometry). 6. week: Mass spectrometric imaging 7. week: Mass Spectrometry coupled with chromatographic methods (GC/MS, HPLC/MS, CZE/MS, TLC/MS) 8. week: Soft ionizaton techniques - quantitative analysis (basic principles of quantitative analysis of molecules using MS, use of MS scans for quantification) 9. week: Soft ionization techniques - qualitative analysis (basic principles of qualitative analysis of organic molecules using MS) 10. week: Spectra Interpretation - soft ionization techniques (Part 1) 11. week: Interpretation of spectra - soft ionization techniques (part 2) 12. week: Analysis of drugs and their metabolites (principles and interpretation of mass spectra) 13. week: Consultation
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The aim of the subject is to provide students with a general overview of the current state of mass spectrometry techniques and to provide them with information on available ionization techniques and mass analyzers, which are closely related to the solution of individual analytical applications. The subject also discusses specific procedures for mass-spectrometric analysis of different groups of synthetic and biological compounds, with an emphasis on the interpretation of their mass spectra, so that students acquire theoretical knowledge in the field and are ready for work on GC/MS, HPLC/MS and on other mass spectrometry systems.
Students should acquire theoretical knowledge to work on GC/MS, HPLC/MS and other mass spectrometry systems
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