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Lecturer(s)
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Bílková Zuzana, prof. RNDr. Ph.D.
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Slováková Marcela, doc. Mgr. Ph.D.
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Course content
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Basic immunology: Structure of immune system. Antigens. Molecules of innate system, cytokines, HLA, complement. Cells of innate system, phagocytosis. Adaptive immune system: B and T cells. Antibody response, B cell receptor, activation. Primary and secondary reaction. T cell response, activation. Therapy of immune system: vaccination, immunological memory. Basic of Imunoanalytical Methods Antigens and antibodies, stuctures, functions and interactions. Principles of preparation of main types of antibodies. Immunoanalytical methods: immunoassays enzymatic, radioizotope, fluorescence, electrophoretic Immunoprecipitation, immunoagglutination, complement fixation. Lydyard Peter: Immunology, Garland Science 2011; chapters A to F. Daussant Jean: Introduction to Immunochemical techniques, Institute of chemical technology Prague 2007; chapters 1 and 2.
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Learning activities and teaching methods
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Dialogic (discussion, interview, brainstorming)
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Learning outcomes
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Annotation: Summary of basic immunology - cells and tissues involved in the immune response, major histocompatibility complex, acquired and specific immune response, humoral and cell-mediated immunity. The basis of clinical immunology focused to the immune and immunopathological reactions (infection, hypersensitivity, organ-specific and systemic autoimmune diseases, immunohematology, transplantation immunology, cancers). Lectures are completed with the theoretical basis of antigen, antibody structure, polyclonal and monoclonal origin of antibodies, theoretical basis of antigen-antibody interaction (non-covalent bond, affinity and avidity, polyclonal and monoclonal antibodies, genetically engineered antibodies). The immunochemistry part is extended by demonstration of methods based on specific Ag-Ab interaction (agglutination, precipitation, immunoblotting, immunofixation, solid-phase immunoassays with various indicators - EIA, RIA, FIA), immunoaffinity chromatography (immunomagnetic separation). Engineering of antibodies and alternative formats applied in practice. The review of immunochemical methods application - medical diagnostics (infection diseases, autoimmune diseases, drugs of abuse, hormones, therapeutics, food safety analysis (quality control, allergens, food born pathogens), environmental testing (pollutant, toxins, heavy metals, pesticides), drug analysis and quality control. Recent trends in immunoanalysis as miniaturization, automatization, and multiplex analyzers. Aim: to obtain basic knowledge of immunology and immunochemistry, the ability to explain immunopathological processes occurred in human body, to understand the principles of all immunoanalytical methods and define the possibilities of their application in practice. Competence: to select proper immunoanalytical method for analysis of biological materials (body liquids, tissues, cell lysates, microorganisms as bacteria or viruses, foods, plants, drugs and therapeutics) and evaluate results in the context of samples origin.
Student will be able to explain mechanisms of immunological defence to bacterias and viruses, and mechanisms of immune system after transplantation and in deficiences estates.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written examination
Written examination
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Recommended literature
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Daussant Jean, F.-Xavier Desvaux. Introduction to immunochemical techniques.. 2007.
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Gabriel Virella. Medical immunology. 2001. ISBN 0-8247-0550-5.
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Janeway, Ch. A. jr. Immunobiology, 6th ed., 2001.
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Klein, Jan; Hořejší, Václav. Immunology, 2nd ed., Oxford : Blackwell Science, 1997.
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Kuby, Janis. Immunology, 2nd ed., New York : W.H. Freeman, 1994.
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Peter Lydyard, Alex Whelan, Michael Fanger. Immunology. 2011. ISBN 978-0-415-60753-7.
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Roitt, I. M. Essential immunology (A), last edition.
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