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Lecturer(s)
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Bílková Zuzana, prof. RNDr. Ph.D.
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Course content
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Summary of basic immunology - cells and tissues involved in the immune response, major histocompatibility complex, acquired and specific immune response, humoral and cell-mediated immunity. The basis of clinical immunology focused to the hypersensitivity reactions, tolerance and autoimmunity, organ-specific and systemic autoimmune disease, immunohematology, immune system modulators, immunodeficiencies, infection of the immunocompetent cells, transplantation immunology, tumor immunology and malignancies of the immune system. All above mentioned sections are complemented by applied diagnostic methods. Lectures are completed with the theoretical basis of antigen, antipody structure, and summary of methods for thin isolation and purification, theoretical basis of antigen-antibody interaction (non-covalent bond, affinity and avidity, polyclonal and monoclonal antibodies, genetically engineered antibodies). The immunochemistry part is extended by demostration of methods based on specific Ag-Ab interaction (SDS-PAGE electrophoresis with immunoblotting, immunoelectrophoresis, immunofixation, solid-phase immunoassays - optimization, verification (EIA, RIA, FIA), immunoaffinity chromatography (immunomagnetic separation), isolation and purification of immunoglobulins, antigens. Engineering antibodies. The review of application of immunochemical methods in clinical practice.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Demonstration
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Learning outcomes
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To obtain basic information about clinical immunology, diseases in context with activity of immune system.
Students know the basics about clinical immunology, diseases of human immune system, also the principle of all the basic tests used in clinical medicine for diagnosis in immunology, microbiology, allergology, transplantation immunology, immunohematology. To predict the complex of diagnostic tests and interpret the results of them.
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Prerequisites
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Student should know the basics from cellular biology, microbiology and immunology.
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Assessment methods and criteria
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Written examination, Home assignment evaluation, Student performance assessment
Check-up of the study - questions at lecture time Final writen exam of the class - 33 % of the exam Oral examination (2 questions) - 67 % of the exam
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Recommended literature
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Ferencik M. Handbook of Immunochemistry. NY: Chapman & Hall, 1993.
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Christine D. Stevens. Clinical Immunology and Serology: A Laboratory Perspective, 2nd edition, 2003.
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Johnstone A., Thorpe R. Immunochemistry in practice, Blackwell Science, 1996.
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Johnstone Alan P., Turner M. W. Immunochemistry 1-practical approach and Immunochemistry 2-practical approach, Oxford : Oxford University Press, c1997.
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Kay A.B. et al. Allergy and allergic diseases. Blackwell Science, 1997.
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Phillips T. M. Analytical Techniques in Immunochemistry, Marcel Dekker inc., 1992.
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Virella G. Medical immunology, 5th ed., rev. and expanded, New York : Marcel Dekker, 2001.
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