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Lecturer(s)
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Kanďár Roman, prof. Mgr. Ph.D.
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Course content
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1. Safety in clinical biochemistry laboratory, laboratory information system (OpenLIMS), administration of laboratory information system, communication of laboratory information system with analyzer, create of batch for analyzer, import results. Analyzer Erba XL200, analyzer operation, liquid chromatograph with spectrophotometric, fluorescence and electrochemical detector, chromatograph operation, gas chromatograph with flame ionization and mass detector, pH meter, pH meter calibration, pH measurement, buffer and mobile phase preparation, Clarity chromatography software from DataApex, evaluation of chromatograms, control of individual modules of HPLC system. 2. Analytical parameters of the method. Determining of precision, accuracy, linearity, limits of detection and quantification. Comparison of 2 methods, comparison of two independent groups by box plots, pairwise comparison of two groups by regression analysis, pairwise comparison of two groups by Bland-Altman plot. 3. Determination of selected antidepressants by gas chromatography with flame ionization detector. Preparation of urine samples before GC analysis - SPE (solid phase extraction) technique. 4. Determination of amino acids in dry blood spot by liquid chromatography with fluorescence detection. 5. Determination of delta-9-tetrahydrocannabinol in plasma by gas chromatography with mass spectrometry detection.
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Learning activities and teaching methods
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Laboratory work
- Practical training
- 40 hours per semester
- Home preparation for classes
- 30 hours per semester
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Learning outcomes
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The aim is to prepare the student for working in laboratories of Clinical Biochemistry.
Student will be able to work in laboratories of Clinical Biochemistry.
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Prerequisites
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Not specified
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Assessment methods and criteria
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Written examination, Student performance assessment
Final written examination
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Recommended literature
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Doležalová V. a spol. Laboratorní technika v klinické biochemii a toxikologii, 1995, IVPZ Brno.
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Doležalová, V. Principy biochemických vyšetřovacích metod. Část I. a II.. 1. vyd. IDVPZ, Brno 1995, 374 s. + 223 s.
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Jaroslav Racek. Klinická biochemie. Praha: Galén, 2006. ISBN 80-7262-324-9.
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Masopust, J. Klinická biochemie I, II, (požadování a hodnocení biochemických vyšetření), Karolinum, 1998.
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Racek, J. a kol. Klinická biochemie, Galén, Karolinum, 1999.
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Štěpánková Š., Královcová P., Kanďár R. Laboratorní cvičení z obecné a klinické biochemie. ISBN 978-80-7395-958-6.
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Zima, T. Laboratorní diagnostika, Galén, Praha, 2002.
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