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Lecturer(s)
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Žáková Pavla, Mgr. Ph.D.
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Kanďár Roman, prof. Mgr. Ph.D.
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Course content
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Practice 1 Clinical Biochemistry laboratory: work safety Analytical parameters of method Concept definition: linearity, accuracy, precision, standard addition method, dilution experiment, robustness, limit of quantification, limit of detection Analytical parameters of selected method: intra-assay, linearity, limit of quantification and detection, accuracy of calibration curve (control serum), statistical evaluation (software Trilobyte, QCExpert), standard addition method, dilution experiment 8 hours Practice 2 Laboratory Information System LISSET: administration; LISANA: communication between LIS and analyzers, import of results Analyzers Cobas Mira and Olympus AU640, operation A comparison of results obtained by analyzers Olympus AU640, Cobas Mira (Roche) and nine-channel photometer Labsystems, statistical evaluation 8 hours Practice 3 The determination of alpha-tocopherol in plasma using high-performance liquid chromatography with coulometric electrochemical detection 8 hours Practice 4 Determination of selected antidepressants by gas chromatography with flame-ionization detector 8 hours
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Learning activities and teaching methods
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Laboratory work
- unspecified
- 40 hours per semester
- unspecified
- 30 hours per semester
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Learning outcomes
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The aim is to prepare the student for working in laboratories of Clinical Biochemistry.
Student will be able to work in laboratories of Clinical Biochemistry.
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Prerequisites
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KBBV/C346 or KBBV/C632 Laboratory of Clinical Biochemistry
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Assessment methods and criteria
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Written examination, Student performance assessment
Final written examination
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Recommended literature
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Doležalová V. a spol. Laboratorní technika v klinické biochemii a toxikologii, 1995, IVPZ Brno.
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Doležalová, V. Principy biochemických vyšetřovacích metod. Část I. a II.. 1. vyd. IDVPZ, Brno 1995, 374 s. + 223 s.
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Jaroslav Racek. Klinická biochemie. Galén, 2006. ISBN 80-7262-324-9.
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Masopust, J. Klinická biochemie I, II, (požadování a hodnocení biochemických vyšetření), Karolinum, 1998.
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Racek, J. a kol. Klinická biochemie, Galén, Karolinum, 1999.
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Zima, T. Laboratorní diagnostika, Galén, Praha, 2002.
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