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Lecturer(s)
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Handl Jiří, RNDr. Ph.D.
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Čapek Jan, Mgr. Ph.D.
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Course content
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1st week: Principles of work in a cell culture laboratory - work safety and hygiene. 2nd week: Spectrophotometric verification of accuracy and correctness of pipetting. 3rd week: Osmotic processes in cell cultivation - plasmoptysis and plasmorhiza of cells. 4th week: Preparation of a native preparation of animal cells, laboratory materials (culture plastic, glass). 5th week: Evaluation of cell viability, cryopreservation and storage of cell cultures. 6th week: Quantification of cell material, analysis of protein concentration. 7th week: Cytochemical and fluorescent staining, visualization of cell organelles. 8th week: Operation of a cell laboratory, principles of work in a laminar flow box, cell isolation. 9th week: Cultivation of suspension cell lines - blood tumor cell lines. 10th week: Cultivation of adherent cell lines - lung and neural tumor cell lines. 11th week: Cultivation of adherent cell lines - liver and kidney cell lines. 12th week: Characteristics of cell growth in the study of cytotoxicity of biomaterials in vitro. 13th week: Presentation of the results of the group project - analysis of cytological study.
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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The aim of the course is to introduce students to the basics of working in a cell culture laboratory and to modern laboratory techniques used in cell analyses.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written examination, Presentation
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Recommended literature
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Pokyny v elektronické podobě dostupné všem studentům v IS STAG..
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ALBERTS, B. a kol. Základy buněčné biologie (Úvod do molekulární biologie buňky). 2. vyd. Ústí nad Labem: Espero, 2005. ISBN 80-902906-2-0.
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Freshney R. I. Culture of Animal Cells (A Manual of Basic Technique and Specialized Applications),. New Jersey, 2010.
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Mozafari M. Handbook of Biomaterials Biocompatibility. 2020.
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