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Lecturer(s)
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Soukup Tomáš, MUDr. Ph.D.
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Bílková Zuzana, prof. RNDr. Ph.D.
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Course content
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Definition of life, organization of living systems. Cell theory (prokaryotic and eukaryotic cells, plant and animal cells) Basic components of cells. The cell nucleus - organization of DNA, function. Nucleic acids, chromosomes. DNA replication and repair. Transcription and translation of genetic information, posttranslational modification. Protein degradation. The cellular membranes - structure, function, transport across biomembranes, osmosis. The endoplasmic reticulum and Golgi apparatus. Cells and immune system. The mitochondrion - structure, function, respiratory chain, electron transport system, ATP, mitochondrial DNA. The cytoskeleton - microtubules, microfilaments, intermediate filaments. Cell motility, cellular motors. Cell reproduction - cell growth and division. Cell cycle and its regulation. Stem cells. Niché. Apoptosis. Necrosis. Regulation of cellular functions in the entire organism. Gene. Genetic code. Cytogenetics and prenatal diagnostics. Mutations. DNA repair. Variability. Malignant transformation. Tumor classification. Human karyotype. Tumor cells in vivo and in vitro. Cell cultures in biomedicine. Tissue engineering.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory work
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Learning outcomes
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This subject is a continuation of subject biology. In seminars, students gain knowledge in cytology of eukaryotic cells and moreover in a progressively developing field of stem cell biology, malignant transformation and regenerative medicine incl. closely related areas of tissue engineering and cell therapy.
In seminars, students gain knowledge in cytology of eukaryotic cells and moreover in a progressively developing field of stem cell biology, malignant transformation and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.
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Prerequisites
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not specified
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Assessment methods and criteria
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Written examination
written exam
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Recommended literature
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Alberts, B. a kol. Základy buněčné biologie. Espero Publishing, Ústí n.L. 2005.
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Masopust, J. a kol. Patobiochemie buňky, Praha, 2003.
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Nečas, O. a kol. Obecná biologie pro lékařské fakulty. H&H, Jinočany 2000.
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