1st week Introduction to cell biology - prokaryotic and eukaryotic cells. Basic building blocks of cells. 2nd week Cell compartmentation - endoplasmic reticulum, Golgi complex, Cytoskeleton - microtubules, microfilaments, intermediate filaments. Cell membranes - structure, function, transport of substances, osmotic phenomena, diffusion. 3rd week Mitochondria - structure, function, ATP production, mitochondrial DNA. 4th week Nucleus - structure, function, nucleic acids, chromosomes, DNA synthesis, gene, genetic code. 5th week Cell death - classification (apoptosis, necrosis, autophagy, senescence). 6th week Cell reproduction - cell cycle and its regulation. Surface structures of cells - adhesive molecules, intercellular connections, protein structures. Stem cells. 7th Malignant transformation of cells - oncogenes, anti-oncogenes, proto-oncogenes. Classification of tumors. Characteristics normal and tumor cells (in vitro, in vivo). 8th week Collection of cytological samples, types of biological material - FNA, smears, swabs, biopsies, aspirations, etc. 9th week Basic cytological examination, cytological screening - examination indications, issues of HGUC, HPV, etc. 10th week Operation of the cytology laboratory - sterility of work, storage of samples. 11th week Working with cells in the cytology laboratory - use of cell cultures in biomedicine, passaging, cell media. 12th week Sample preparation for cytological analysis, types of staining, bioanalytical determinations, evaluation. 13th week In vitro fertilization, prenatal screening. Personalized medicine.
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Alberts, B. a kol. Základy buněčné biologie. Espero Publishing, Ústí n.L. 2005.
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Freshney R.I. Culture of animal cells (A manual of basic technique and specialized application). 2015.
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Masopust, J. a kol. Patobiochemie buňky, Praha, 2003.
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Mather J. P. a Roberts P. E. Plenum press Introduction to Cell and Tissue Culture (Theory and Technique). 1998: New York and London. ISBN 0-306-45859-4.
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