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Lecturer(s)
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Šmíd Lenka, Mgr. Ph.D.
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Roušar Tomáš, prof. RNDr. Ph.D.
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Soukup Tomáš, MUDr. Ph.D.
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Handl Jiří, RNDr. Ph.D.
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Course content
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Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research. Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors. Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation. Tissue engineering. Niche - microscopy. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy. Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages Orthopaedic applications. Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury. Muscle-derived stem cells; muscle regeneration Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics. Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation. Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis Visit of tissue culture laboratory. Cell viability. Preparation of cells for flow cytometric analysis.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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Students who have passed cytology are eligible to register. In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.
In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.
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Prerequisites
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cytology and cytodiagnostics
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Assessment methods and criteria
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Written examination, Student performance assessment
Presence in lectures and laboratory classes. Absences may be substituted only by writing an assay on the topic you missed. Elaboration of protocol for cell viability and presentation for other students. Final examination in written form of multiple choice test.
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Recommended literature
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Doyle et al. Mammalian Cell Culture (Essential Techniques), John Wiley and Sons, 1997. ISBN 0-471-97057-3.
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Filip, S., Mokrý, J., Hruška, I. Kmenové buňky - Biologie, medicína, filozofie, Galén, Praha 2006.
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Lanza et al. Principles of Tissue Engineering, Academic Press, 2000. ISBN 0124366309.
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Masopust, J. a kol. Patobiochemie buňky. Praha, 2003.
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Turksen, K. Adult Stem Cells, Humana Press, 2004. ISBN 1-59259-732-7 7.
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