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Lecturer(s)
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Vydržalová Markéta, RNDr. Ph.D.
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Pejchalová Marcela, doc. Ing. Ph.D.
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Course content
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The laboratories run in three blocks. Each block lasts one week (10 h) during which students process an unknown clinical specimen under conditions simulating normal clinical practice. The result of the laboratory work is to identify the causative agent of the infectious disease. Block 1: Skin and soft tissue infections. Block 2: Respiratory tract infections. Block 3: Infections of the gastrointestinal and urogenital tract.
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Learning activities and teaching methods
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Methods of individual activities, Laboratory work
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Learning outcomes
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The main goal of the laboratory practise is to acquaint students with direct and indirect diagnostic procedures used to determination of infectious disease agents. Students are informed about the importance of proper collection and transport of biological material and problems associated with non-compliance with these principles. It also includes information on work safety and good laboratory practice. In laboratory pracice taking place in three week blocks (10 h), the students, on the basis of the anamnestic data presented in the accompanying sheet, independently perform the examination of biological material in order to detect the infectious agent. Diagnostics includes direct microscopy, cultivation on appropriate culture media, identification of the agent based on morphological, growth, biochemical and antigenic properties. It includes determination of sensitivity to antibiotics. Finally, the student performs a clinical-microbiological evaluation of the finding.
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Prerequisites
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Necessary is to know the basic genera, morphology and arrangement of bacterial cells, the character of growth on culture media and the results of basic biochemical tests to identify an unknown strain.
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Assessment methods and criteria
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Home assignment evaluation
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Recommended literature
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BENEŠ, J. Infekční lékařství. 1. vyd. Praha: Galén, 2009. ISBN 978-80-7262-644-1.
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Mosio, Petra. Atlas bakterií. Pardubice: Univerzita Pardubice, 2012. ISBN 978-80-7395-467-3.
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Votava, M. Lékařská mikrobiologie obecná. NEPTUN, Brno 2001, 231 s.
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Votava, M. Lékařská mikrobiologie speciální. NEPTUN, Brno 2003, 453 s.
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Votava, Miroslav. Lékařská mikrobiologie vyšetřovací metody. Brno: Neptun, 2010. ISBN 978-80-86850-04-7.
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