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Lecturer(s)
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Královec Karel, RNDr. Ph.D.
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Course content
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Principles of health and safety in biological laboratory. Work with light microscope. Methods for increasing of the contrast of views in light microscopy (dark field, phase contrast, DIC). Preparing of native preparation. Vital dying, plant cells and organelles. Mitosis, dying, observation of chromosomes. Meiosis. Pernament preparation, blood slide, panoptic dying. Morphology of animal cells. Differential leukocytes counts. Cytometry-measuring of cells sizes. Counting of cells. Biochemical urine test, microscopic examination of the urinary sediment. Plant tissues. Video: ultrastructure of plant and animal cells, mitosis, meiosis, and cytokinesis
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory work
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Learning outcomes
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The emphasis is in particular on a perfect mastery of the work with the light microscope, including special microscopic methods (dark field, phase contrast, Nomarski differential interference contrast).
Students will be able to work with light microscope and prepare microscopical preparations.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Written examination
Written exam. Students must answer exactly at least 60 % questions.
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Recommended literature
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Návody jsou k dispozici ve STAGu.
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Berger J., Holinka J. Základní biologická cvičení. Skriptum Univerzity Pardubice 1995.
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Hejtmánek M. Úvod do světelné mikroskopie. Skriptum UP Olomouc 2001. 2001.
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Paleček J. Biologie buňky. I. Základy mikroskopické cytologie. Vydavatelství Karolinum, Praha 1996.
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