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Lecturer(s)
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Jankovičová Barbora, Mgr. Ph.D.
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Řehulka Pavel, Mgr. Ph.D.
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Course content
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unspecified
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Projection
- Writing a seminar paper
- 5 hours per semester
- Excursion
- 2 hours per semester
- Term paper
- 10 hours per semester
- Home preparation for classes
- 70 hours per semester
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Learning outcomes
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Part of the subject is familiarization with sample preparation procedures for proteomic analysis, analytical and preparative methods, including specific analysis outputs and their interpretation. It also includes a demonstration of the introduction of new analytical methods into clinical practice and the use of methodological potential to search for new diagnostically significant markers of specific processes in biological materials. The lectures will include thematic blocks dealing with the separation of protein mixtures (conventional chromatography, affinity chromatography, electrophoretic methods including 2D arrangement), identification of selected proteins by various methods, with an emphasis on mass spectrometry using basic proteomic databases. Students will learn the basics of peptide mapping and how to work with the tools used for this purpose (Mascot, Protein Prospector, etc.). The thematic block dedicated to the ionization techniques including instrumentation, tandem mass spectrometry with the possibility of sequential peptide analysis, the combination of HPLC with MS detection, and quantitative analysis procedures using MS. The final thematic block is devoted to current applications in the field, which find application in practice, e.g. in diagnostics in clinical practice.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination, Written examination, Home assignment evaluation
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Recommended literature
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Jörg Reinders, Albert Sickmann. Proteomics. Methods and Protocols. Methods in Molecular Biology,. Humana Press, 2009.
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J.R. Chapman. Mass Spectrometry of Proteins and Peptides. Humana Press, 2002.
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O. Geshke, H. Klank, P. Telleman. Microsystem Engineering of Lab-on-chip Device. Weinheim, 2005.
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R. Westermeier, T. Naven. Proteomics in practice. Wiley-VCH: Weinheim.
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