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Lecturer(s)
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Slováková Marcela, doc. Mgr. Ph.D.
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Bílková Zuzana, prof. RNDr. Ph.D.
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Korecká Lucie, doc. RNDr. Ph.D.
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Course content
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The theoretical basis of antigen-antibody interaction (non-covalent bond, affinity and avidity, polyclonal and monoclonal antibodies, genetically engineered antibodies). The theoretical basis with practical training of methods based on specific Ag-Ab interaction (SDS-PAGE electrophoresis with immunoblotting, immunoelectrophoresis, immunofixation, solid-phase immunoassays optimalization, verification (EIA, RIA, FIA), immunoaffinity chromatography (immunomagnetic separation), isolation and purification of immunoglobulins, antigens. Engineering antibodies. The review of application of immunochemical methods in clinical practice.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Skills training, Laboratory work
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Learning outcomes
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The aim of this subject is to develop the skills necessary to become an expert in immunochemical arrays for analysis of biological materials in clinical diagnostics. The general goal is to develop the student in a flexible fashion adapted to the requirements of analytical practice. Laboratory training in a wide range of immunochemical techniques, in their validation and precise interpretation, preparation of reagents and complex kits is also considered part of the educational program.
Student will obtain good knowledge of all methods used for analysis of biological material, clinical material, food, methods based on the biospecific reaction between antigen and specific antibody. Student know how to install the test in laboratory, how to optimize the condition and validate the test for commercial application.
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Prerequisites
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Student should know the basics from cellular biology, microbiology and immunology. C636 Immunology C627 Biochemistry
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Assessment methods and criteria
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Written examination, Student performance assessment
Laboratory protocols (30 %), written test (70 %).
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Recommended literature
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Bartůňková J, Paulík M. Vyšetřovací metody v imunologii, Grada, 2005.
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Johnstone A., Thorpe R. Immunochemistry in practice, Blackwell Science, 1996.
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Phillips T. M. Analytical Techniques in Immunochemistry, Marcel Dekker inc., 1992.
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