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Lecturer(s)
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Šilha David, doc. Ing. Ph.D.
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Course content
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The principle of the work in microbiology lab, safety, equipment, desinfection, aseptic work, preparation of glassware. Microscopy. Native and simple staining, stable praparation. Diferentia and diagnostic staining of bacteria. Gram staining, negative staining. Staining of spores and capsules. Staining of volutine, proteins and nucelus in yeasts. Determination of the size of microorganisms. Preparation and effusion of nutrient bases. Inoculation, isolation of pure culture, storage and reviving of bacteria. The growth of microorganisms on the solid and liquid media. Growth curve. Identification of yeasts, blastospores and pseudomycelium. Microscopy of fungi, preparation of glass-slide culture. Determination of mobility of bacteria and the concentration of cells using Burker chamber. Substitute lab, written exam.
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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The objective is to acquaint with the work´s principle and rules in microbiology lab, prepare various kind of microscopic preparations and nutrien media for growing of microorganisms. Students adopted various techniqes of inoculation and cultivation of various species and will be able to evaluate their characteristics.
Students govern a basic principle of microbiological work (aseptic), can prepare nutrient media and inoculate and cultivate various kind of microorganisms.
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Prerequisites
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KBBV/C604 - Optical microscopy techniques in health service
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Assessment methods and criteria
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Written examination, Student performance assessment
A credit is given upon the graduation of all the experiements, preparation of protocols and final check test.
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Recommended literature
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Šilhánková L., Demnerová K. Návody pro laboratoře z mikrobiologie. SNTL Praha, 1985.
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Vytřasová, J., Bílková Z. Laboratorní cvičení z obecné mikrobiologie. Univerzita Pardubice, 1998.
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