Fundamentals of immunochemistry, the reaction of an antigen (analyte) with a specific antibody, the use of this bioaffinity highly specific and sensitive reaction as part of analytical, detection or diagnostic tests Structure of antigen, antibodies, including their preparation, production, quality control Methods of isolation and purification (fractional precipitation, electrophoresis, liquid chromatography, magnetic separation, ultracentrifugation, etc.) of reagents used for immunochemical methods Antigenicity, immunogenicity, hapten, determinant, epitope, affinity, avidity Agglutination reaction, hemagglutination reaction, precipitation, immunodiffusion reaction, immunoelectrophoresis, EIA, RIA, FIA, ECL, blotting techniques, affinity chromatography methods, immunosensors Immunochromatographic tests - clearview, "rapid" tests, "bedside" tests, "quick" tests, etc. Principles of POCT - point-of-care tests Practical examples of the use of IA methods in practice: clinical diagnostics, analysis of food, water, microbiology, veterinary medicine, etc. Construction of immunoanalytical methods, binding and modification of antibodies, antigens New generation immunoanalyzers: protein chips, fluidic immunosensors, electrochemical immunosensors, methods based on SPR, SELDI, etc.
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Elektronické podklady, k dispozici na studijním webu university (STAG).
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