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Lecturer(s)
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Komers Karel, prof. Ing. CSc.
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Komersová Alena, doc. Ing. Ph.D.
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Course content
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Definition of biophysical chemistry. Classification and organization of biosystems, differentiation from lifeless systems. Physical and chemical methods of study of biomoleculs and their groupage up to the level of biopolymers. Structure and properties of biopolymers: proteins, nucleic acids, polysacharides and lipids. Biomembranes. Biothermodynamics: thermodynamic specialities of biosystems. Entropy, orderliness and informations. Irreversible thermodynamics and biosystems. Bases of enzymology: classification, terminology, structure and forms of existence of enzymes. Kinetics of biochemical processes: Enzymatic reactions - Michaelis-Menten equation, kinetics with two or more substrates, kinetics of inhibition. Kinetics of grow (of number and/or mass) of microorganisms and substrate kinetics in the batch reactor and chemostat. Bases of pharmacokinetics: kinetics of fast intravascular and extravascular dosage of drug.
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Learning activities and teaching methods
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Work with text (with textbook, with book)
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Learning outcomes
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The students obtain the knowledge about 1) classification and properties of biosystems up to the level of biopolymers in terms of physical chemistry, 2) analytical methods making possible the qualitative and quantitative description of these biosystems and 3) application of thermodynamics and reaction kinetics on these biosystems. Graduates of this course will be able to solve independently the physico-chemical problems from the sphere of biothermodynamics (e.g. calculations of energetic changes of bioreactions) and kinetics of processes proceeding in biosystems (e.g. determination of the reaction scheme and rate constants of enzymatic and pharmacokinetic reactions).
The graduate of this object will be able to solve independently the physico-chemical problems from the region of biothermodynamics (e.g. calculations of energetic changes of bioreactions)and kinetic processes running in biosystems (e.g. determination of reactin schemes andrate constants of enzymatic and pharmacochemical reactions.
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Prerequisites
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Successful graduation of the basic courses of mathematics, physics, general and anorganic, organic, analytic and physical chemistry (especially thermodynamics and chemical kinetics)
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Assessment methods and criteria
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Oral examination, Written examination
In the course of the semester two written test will be submited (examples and/or questions), the classification makes 0 - 40% from the whole. The exam is written (0-20%) and oral (0-40%). The object is graduated if minimum 50% is reached. The ignorance of one question at the oral exam means the failure of the whole exam regardless of the previous results.
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Recommended literature
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Devlin T. M. Texbook of Biochemistry, Willey-Liss, 3. edition 1992.
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Kodíček M., Karpenko V. Biofyzikální chemie, Academia Praha 1977.
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Komers K., Čegan A., Komersová A., Machek J. Biofyzikální chemie. Univerzita Pardubice, 2003.
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Kotyk A., Horák J. Enzymová kinetika, Academia Praha 1977.
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Vodrážka Z. Fyzikální chemie pro biologické vědy, Academia Praha 1982.
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