Course: Fundamentals of Laboratory Techniques

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Course title Fundamentals of Laboratory Techniques
Course code KOANCH/C224
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Semester Winter
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Hostinský Tomáš, Ing. Ph.D.
  • Kucek Vladimír, Ing. Ph.D.
  • Jambor Roman, prof. Ing. Ph.D.
  • Pálka Karel, doc. Ing. Ph.D.
  • Ruleová Pavlína, Ing. Ph.D.
  • Vinklárek Jaromír, prof. Ing. Dr.
  • Schwarz Jiří, Ing. Ph.D.
  • Střižík Lukáš, Ing. Ph.D.
Course content
1st week: introduction, organizational instructions, distribution to tables, issuance of protective equipment, work safety, weighing, volume measurement, preparation of soluble inorganic salt solutions. 2nd week: filtration, vacuum in the laboratory, drying of substances, lyophilization, preparation and isolation of insoluble carbonates 3rd week: solubility of substances, crystallization, cooling in the laboratory, storage of solid substances, types of crystallization, recrystallization of the presented substances. 4th week: sublimation, distillation, p-t diagram, use of vacuum, used laboratory technique, sublimation and distillation under atmospheric pressure. 5th week: redox reactions, dissolution and excretion of metals, nobility of metals, transformations of copper (Cu Cu(NO3)2 CuO CuSO4 Cu) (preparation in small quantities) Week 6: neutralization, general methods of preparing salts, concept of pH, pH of strong acids and bases, acid-base indicators, preparation of salts by neutralization and their isolation from solution, control test (30 min.). 7th week: electrolytic dissociation, pH of weak acids, salts and buffers, glass pH electrode, determination of dissociation constant of acetic acid, determination of buffer pH 8th week: titration curves, back titration, antacids, determination of neutralizing capacity of antacids 9th week: chromatographic separation methods, experimental conditions of paper chromatography, separation of some metal cations by paper chromatography. 11th week: photosynthesis, chlorophyll, carotenes, experimental conditions of thin-layer chromatography (silufol), extraction and chromatographic separation of plant dyes Week 12: simple sugars, structure, chemical properties, reactions of monosaccharides and disaccharides with Fehling's reagent 13th week: Substitutes for laboratory exercises, Final written work. The written work consists of a calculation part (examples for the preparation of solutions, simple stoichiometry and crystallization, corresponding in difficulty to calculations in laboratories) methodological parts (concepts, apparatus drawings, diagrams, basic laboratory operations, laboratory preparation equations). Week 14: Conclusion of laboratory exercises - analysis of errors in the final written work, individual evaluation of students, awarding of credit.

Learning activities and teaching methods
Demonstration, Laboratory work
  • Preparation for a final test - 10 hours per semester
  • Home preparation for classes - 14 hours per semester
  • Practical training - 42 hours per semester
  • Preparation for laboratory work and processing of results - 14 hours per semester
Learning outcomes
The main goal is the acquisition of basic laboratory techniques and the verification of theoretical knowledge, which students were introduced to in the lectures of the Basics of Inorganic Chemistry course.
After completing the course, the student is able to calculate laboratory examples focused on the preparation and dilution of solutions, crystallization and stoichiometry. To prepare simple inorganic compounds using basic laboratory operations and laboratory technology.
Prerequisites
Basic theoretical and computational knowledge of general and inorganic chemistry in the scope of a high school graduate.

Assessment methods and criteria
Written examination, Home assignment evaluation, Systematic monitoring

In the course of the laboratories, the preparation is checked by checking the laboratory diaries, continuous written tests and oral examinations of individual students. At the end of the laboratories, the students hand over the resulting products of their experimental work for inspection, which they will process in writing in the form of a protocol for the next laboratories. Experimental results, interim control results and final written exams are included in the overall assessment.
Recommended literature
  • Handlíř, Karel. Laboratorní cvičení z obecné a anorganické chemie I. Pardubice: Univerzita Pardubice, 2021. ISBN 978-80-7560-370-8.
  • Handlíř, Karel. Výpočty a cvičení z obecné a anorganické chemie I. Pardubice. 2021.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester