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Lecturer(s)
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Mikulášek Petr, prof. Ing. CSc.
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Course content
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Biotechnology - an interdisciplinary pursuit. A contribution of Bioengineering to Biotechnology. What is Bioengineering? Kinetics of enzyme-catalyzed reactions. Simple enzyme kinetics with one and two substrates. Kinetics of substrate utilization, product yield, and biomass production in cell cultures. Overall kinetics in cases of chemical-reaction-mass-transport interaction. Design and analysis of biochemical reactors. The ideal continuous-flow stirred-tank reactor. The ideal plug-flow tubular reactor. Residence-time distributions. Dispersion models for nonideal reactors. Transport phenomena in microbial systems. Bioreactors. Microbial bioreactors. Non-microbial cell culture systems. Enzyme bioreactors. Relationships between batch and continuous biological reactors. General description of stirred vessel bioreactor. Mechanics of fluid flow and gas dispersion in stirred vessel. Experimental design, parameter optimatisation, scaling-up. Bubble column bioreactors. Hydrodynamics. Models of bubble columns. Biochemical loop reactors - propeller (PLR) and air-lift loop reactors (ALR). Bioreactors second generation. Immobilized cell/enzyme bioreactors. Membrane bioreactors. Downstream processing operations. Precipitation. Crystallization. Centrifugation. Membrane separations. Chromatographic methods in bioprocessing. Supercritical fluid extraction. Special problems of bioreactors. Bioreactor and catalyst design for fermentation processes. Costs. Sterilization and preservation in bioprocessing, safety in biotechnology. Fundamentals of computer control in fermentation processes. Biotechnology in waste water treatment and environmental technologies.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Methods of individual activities
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Learning outcomes
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The course covers the principles of biotechnologies from a chemical engineering point of view. The emphasis is given on examining the systems and components that are common to most or all biotechnology production processes.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination, Written examination
final project - 60% oral exam - 40%
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Recommended literature
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Doran P.M. Bioprocess Engineering Principles. Academic Press Limited, London, 1995.
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Lydersen B.K., D'Elia N.A., Nelson K.M. Bioprocess Systems, Equipment and Facilities. John Wiley & Sons, 1994.
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Ratledge C., Bjorn K. Basic Biotechnology. Cambridge Univ. Press, 2001.
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