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Lecturer(s)
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Cuhorka Jiří, Ing. Ph.D.
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Šiška Bedřich, Ing. CSc.
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Palarčík Jiří, Ing. Ph.D.
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Course content
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1. Safety and principles of laboratory work, laboratory equipment, methods of sterilization and disinfection, work with toxic substances, sources of UV radiation, principles of aseptic work, basic terminology, glass preparation. 2. Preparation and pouring of nutrient media. Practice of inoculation using various techniques, isolation of pure culture, preservation and revival of bacterial cultures. 3. Use of microscopic analyzes in water technology. Microscope. Principles of VIS microscopy. Permanent preparations, native preparation, simple staining. 4. Gram´s staining procedure, negative staining. 5. Determination of CFU in a sample, determination of cell number in a Bürker chamber. 6. Determination of bacterial growth curve. 7. Membrane bioreactor 8. Ozonization 9. Photolysis 10. Photocatalysis - process characteristics - catalyst (type, concentration) 11. Photocatalysis - process characteristics - influence of matrices (pH, ions, organic substances) 12. Photocatalysis - UV source characterization 13. Completion of missing laboratory exercises; final test.
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Learning activities and teaching methods
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Methods of individual activities, Laboratory work
- Practical training
- 39 hours per semester
- Preparation for laboratory work and processing of results
- 52 hours per semester
- Preparation for a final test
- 20 hours per semester
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Learning outcomes
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The aim of the course is to acquaint students in practical terms on model devices with the basic processes that are discussed in the course Biological and Advanced Degradation Processes. Students determine the basic characteristics of processes and become familiar with the possibilities of influencing them by changing the conditions of the experiment.
Students will learn the methodology of experimental study of processes. After completing the course, they are able to verify and compare the efficiency of degradation processes and determine their basic operational characteristics.
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Prerequisites
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completion or concurrent study of the course Biological and Advanced Degradation Processes
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Assessment methods and criteria
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Written examination, Home assignment evaluation
Emphasis is placed on practical realization of individual laboratory exercises and processing of laboratory protocols. The extent of acquired knowledge, concepts and application skills from the course of laboratories is verified by a final test.
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Recommended literature
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Katoh, Shigeo. Biochemical engineering : a textbook for engineers, chemists and biologists. Weinheim: Wiley-VCH, 2009. ISBN 978-3-527-32536-8.
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Oppenländer, Thomas. Photochemical purification of water and air. Weinheim: Wiley-VCH, 2003. ISBN 3-527-30563-7.
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