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Lecturer(s)
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Šilha David, doc. Ing. Ph.D.
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Course content
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Microbial cell (structure of prokaryotic and eukaryotic cells). Planktonic and biofilm forms of cells. Microbial biofilm (formation process, composition, structure). Live vs. dead microbial cells, VBNC forms. Experimental methods of biofilm detection - phenotypic methods (spectrophotometry) . Experimental methods of biofilm detection - genotypic methods (PCR, FLU microscopy). Use of intercalation dyes to determine cell viability.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book)
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Learning outcomes
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The course introduces students to the issue of microbial biofilms and the impact of their presence in healthcare and industry.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination, Home assignment evaluation
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Recommended literature
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DINCER, S., ÖZDENEFE, M. S., ARKUT, A. Bacterial Biofilms. London: IntechOpen, 2020. ISBN 978-1-83968-819-5.
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RATHER, M. A., GUPTA, K., MANDAL, M. Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies. Brazilian Journal of Microbiology 2021, roč. 52, č. 4, s. 1701-1718.
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RULÍK, M., HOLÁ, V., VOTAVA, M. Mikrobiální biofilmy.. Olomouc: Univerzita Palackého v Olomouci, 2011. ISBN 978-80-244-2747-8.
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