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Lecturer(s)
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Roušar Tomáš, prof. RNDr. Ph.D.
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Course content
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Oxidative metabolism of cells and its analysis. Mitochondrial respiration. Antioxidant system of cells and its detection. Possibilities of 3D cell culture. Transformation of cell lines, GMOs. Fluorescence microscopy, confocal microscopy.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Methods of individual activities
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Learning outcomes
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination
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Recommended literature
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BALVAN J. Buněčná smrt.. Praha: Grada, 2021. ISBN 978-80-271-1260-9.
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FRESHNEY R.I. Culture of animal cells: a manual of basic technique and specialized application. Hoboken: Wiley, 2021. ISBN 978-1-119-51304-9.
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HALLIWELL, B., GUTTERIDGE, J. M. C. Free Radicals in Biology and Medicine. Oxford: Oxford University Press, 2015. ISBN 978-0-19-871748-5.
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NICHOLLS, D. G., FERGUSON, S. J. Bioenergetics.. 4th ed. Amsterdam: Elsevier Academic Press, 2013. ISBN 978-0-12-388425-1.
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ŠEBEK, J. Buněčné kultury v medicíně.. Praha: Galén, 2018. ISBN 978-80-749-2380-7.
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