| Course title | Mass Spectrometry in Organic Analysis |
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| Course code | KALCH/C015A |
| Organizational form of instruction | Lecture |
| Level of course | Master |
| Year of study | not specified |
| Semester | Winter and summer |
| Number of ECTS credits | 5 |
| Language of instruction | English |
| Status of course | unspecified |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Course availability | The course is available to visiting students |
| Lecturer(s) |
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| Course content |
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Introduction to mass spectrometry (MS): definition of basic terms, instrumentation for mass spectrometry (various ways of sample introduction, separation of ions and their detection, vacuum system), ionization techniques (electron ionization, chemical ionization, field ionization or desorption, fast atom and ion bombardment, thermospray ionization, electrospray ionization, atmospheric pressure chemical ionization, matrix-assisted laser desorption/ionization, etc.), analysers (magnetic analyser and double focussing sector analyser, quadrupole, ion trap, time-offlight and ion cyclotron resonance analysers), tandem mass spectrometry (MS/MS, MSn, basic types of scans), hyphenations with separation techniques (GC/MS, HPLC/MS, CE/MS, SFC/MS), bioorganic mass spectrometry. Principles of fragmentation (?-, i-, ?-cleavages and McLafferty rearrangement), detailed interpretation of conventional electron ionization mass spectra, fragmentation paths for particular compound classes, basic rules of interpretation (isotopic ratios for common organic elements, determination of molecular ion, characteristic ionic series, nitrogen rule, ions with odd and even number of electrons). Interpretation of "soft" ionization mass spectra, collision induced dissociation mass spectra, mass spectra of peptide, proteins and oligonucleotides.
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| Learning activities and teaching methods |
| Monologic (reading, lecture, briefing) |
| Learning outcomes |
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Students will have a general overview of current state-of-art in mass spectrometric techniques including the types of ionization techniques, mass analyzers and coupling with separation techniques. The important part of this course is the interpretation of mass spectra obtained both with electron ionization and soft ionization techniques. Students should obtain the theoretical knowledge suitable for the subsequent work on GC/MS, HPLC/MS and other mass spectrometric systems.
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| Prerequisites |
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Basic knowledge of chromatographic techniques.
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| Assessment methods and criteria |
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Oral examination, Written examination
Basic knowledge of chromatographic techniques. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester |
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