| Course title | Analytical Instrumental Methods |
|---|---|
| Course code | KALCH/C023 |
| Organizational form of instruction | Lecture + Seminar |
| Level of course | Bachelor |
| Year of study | 3 |
| Semester | Winter |
| Number of ECTS credits | 5 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Potentiometry. Electrodes and cells. Glass pH-measuring electrode, experimental approach to measurement of pH. Ion-selective electrodes. Nikolsky-Eisenman equation. Electrodes with membranes. Experimental setups of potentiometric titration, evaluation of titration curves. Polarography/voltammetry. Basics of polarographic measurements. Mercury drop electrode and its properties. Sample preparation for polarographic measurement. Recent trends in voltammetry. Types of titration curves, their interpretation and applications. Electrogravimetry and coulometry. Conductometry and conductometric titrations. Basic approaches and instruments in optical emission spectrometry. Flame photometry. Atomic absorption spectrometry (AAS). Basic setup of atomic absorption spectrometer. Flame atomisation and eletrotermical atomisation. Calibration and applications of AAS. Molecular absorption spectrometry in UV/VIS range. Basic photometric quantities. Bouguert-Lambert-Beer law. Possibilities of identification of compounds. Quantitative analysis. Photometric (micro)titrations. Luminiscence spectrometry. Principles of infrared and Raman spectrometry. Nonspectrometric optical methods. Polarimetry, refractometry, interferometry. Nephelometry and turbidimetry. Extractions, extraction equilibria, definition of equilibrium constants. Extraction of solid compound by liquid and supercritical fluid. Extraction from liquid to liquid, extraction of neutral chelates, extraction of ionic associates. Solid phase extraction, extraction by gas. Chromatography. Basic principles, classification of chromatography according to the mechanism of separation, introduction of sample etc. Processing of chromatographic data. Qualitative and quantitative analysis. Gas chromatography. Liquid chromatography. Paper and thin-layer chromatography. Electromigration separation methods. Electrophoresis and isotachophoresis.
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| Learning activities and teaching methods |
| Monologic (reading, lecture, briefing) |
| Learning outcomes |
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The aim of the course is to extend knowledge of classical analytical methods with basic knowledge about instrumental analytical methods.
Students after graduation of the subject "Analytical instrumental methods" together with the subject C024 "Laboratory of analytical instrumental methods" are competent to execute the simpler procedures in analytical laboratories equipped with the basic accessories |
| Prerequisites |
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High school knowledge of analytical chemistry
KALCH/C024 and KALCH/C019 |
| Assessment methods and criteria |
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Oral examination, Written examination
Current check of study - 2 written tests - 30 % of grade, written part of examination - 20 %, oral examination - 50 %; |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Chemical Technology | Study plan (Version): Chemistry and Technical Chemistry (2016) | Category: Engineering chemistry and chemistry of silicates | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Evaluation and Analysis of Foodstuffs (2015) | Category: Food industry and food industry chemistry | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Chemistry and Technical Chemistry (2013) | Category: Engineering chemistry and chemistry of silicates | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Evaluation and Analysis of Foodstuffs (2014) | Category: Food industry and food industry chemistry | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Evaluation and Analysis of Foodstuffs (2016) | Category: Food industry and food industry chemistry | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Chemistry and Technical Chemistry (2015) | Category: Engineering chemistry and chemistry of silicates | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Evaluation and Analysis of Foodstuffs (2013) | Category: Food industry and food industry chemistry | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Chemistry and Technical Chemistry (2014) | Category: Engineering chemistry and chemistry of silicates | 3 | Recommended year of study:3, Recommended semester: Winter |