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Lecturer(s)
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Hájek Tomáš, Ing. Ph.D.
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Husáková Lenka, doc. Ing. Ph.D.
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Švecová Blanka, Ing. Ph.D.
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Metelka Radovan, Ing. Ph.D.
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Fischer Jan, doc. Ing. CSc.
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Adam Martin, doc. Ing. Ph.D.
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Bartoš Martin, Ing. CSc.
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Česla Petr, doc. Ing. Ph.D.
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Mikysek Tomáš, Ing. Ph.D.
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Course content
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Liquid chromatography - gradient elution. IR spectrometry - identification of samples using IR spectra and quantitative measurements. Stripping voltammetry - determination of heavy metals using carbon paste electrodes with mercury film. Presssurised solvent extraction/gas chromatography - analysis of oils. Flame AAS - determination of calcium and magnesium in mineral water and zinc in red wine samples. Thin layer chromatography - determination of cholesterol, analysis of dyes. Izotachophoresis - determination of anions. Trace analysis by HPLC - determination of trace concentrations of herbicides in water. Trace analysis of mercury - determination of mercury in various matrices by cold vapor method. Gas chromatography - analysis of spirits, quantitative analysis. UV spectrophotometry - interpretation of spectra, quantitative measurements. Micellar electrokinetic chromatography - determination of caffeine in drugs and beverages. Microcolumn chromatography - applications of microcolumns, influence of working conditions on analysis. Capillary gas chromatography - measuring column efficiency, the Kovats retention index system.
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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The aim of this course is to acquaint students with modern laboratory methods of inorganic and organic instrumental analysis, that may be encountered in practice. Laboratory is equipped with appropriate instrumentation for processing and analyzing of samples and computers for collecting and evaluating experimental data.
Students will be able to solve real problems in analytical laboratory equipped with modern instrumental apparatuses.
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Prerequisites
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Knowledges of analytical, physical (and organic) chemistry on the bachelor degree level.
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Assessment methods and criteria
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Written examination, Student performance assessment
Preparation, analysis results, protocol, written exam.
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Recommended literature
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Barek J. (Zýka J., editor). Nové směry v analytické chemii, 1989, SNTL Praha.
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Foret F., Křivánková L., Boček P. Capillary zone electrophoresis.
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Churáček J. a kol. Analytická separace látek, 1990, SNTL Praha..
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Churáček J. a kol. Nové trendy v teorii a instrumentaci vybraných analytických metod. Praha, 1993.
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Zýka J. (ed.). Analytická příručka, díl I a II. Praha, 1988.
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