Course: Atomic Spectrometry

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Course title Atomic Spectrometry
Course code KALCH/C571
Organizational form of instruction Lecture + Seminary
Level of course Master
Year of study 1
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Husáková Lenka, doc. Ing. Ph.D.
Course content
1st week: Introduction to atomic spectrometry (AS): EMG radiation, its properties and interaction with matter, laws of the formation of atomic spectra, probability of absorption and emission, spectral line properties, and classification of AS methods. 2nd week: Atomic absorption spectrometry (AAS): principles, method classification, instrumentation (radiation sources, optical system, atomizers, detectors, signal processing, systems for correction of non-specific absorption). 3rd week: Flame (FL) atomization AAS, electrothermal (ETA) AAS - description of atomization, isothermicity of the atomization process, temperature program and its optimization, matrix interferences - classification, mechanisms, identification and elimination possibilities, STPF concept. 4th week: Generation of volatile compounds: Hydride technique - principles, importance, use, possibilities of hydride generation, transport, separation, collection and atomization, generator arrangement, matrix interferences. Cold vapour method and thermooxidative mercury determination - principles, instrumentation, analytical characteristics, applications. 5th week: Atomic fluorescence spectrometry (AFS): principles, types of fluorescence transitions, instrumentation, matrix interferences, analytical applications. Introduction to optical emission spectral analysis (OES): principles, excitation sources, optical system - arrangement, monochromators and polychromators, detection. 6th week: Flame photometry: theoretical foundations, main parts of the photometer, and use in real applications. Optical emission spectrometry with inductively coupled plasma (ICP-OES): Formation and physical properties of plasma, modern instrumentation, matrix interferences, analytical characteristics, and applications. 7th week: Optical emission spectrometry with microwave-induced plasma (MP-OES), laser-induced breakdown spectroscopy (LIBS), OES with glow discharge (GD-OES) - basic principles and analytical characteristics, instrumentation, matrix interferences, possibilities for quantitative and qualitative element analysis. 8th week: Inductively coupled plasma mass spectrometry (ICP-MS): principles, construction of spectrometers, mass analyzers, resolving power, sample introduction systems, matrix interferences and their removal, analytical characteristics and applications. 9th week: Spectroscopy of inner electrons: principles, primary and secondary excitation, relaxation process, X-ray radiation, labelling of X-ray spectral lines, summary of methods. 10th week: X-ray fluorescence spectrometry (XRF): characteristic radiation and its monochromatization, detection, evaluation of XRF spectra, wavelength dispersive (WDXRF) and energy dispersive (EDXRF) systems and their comparison, qualitative and quantitative analysis, and matrix interferences. 11th week: Selected methods of local analysis and surface analysis: overview, principles, arrangement, significance, and use. 12th week: Element speciation using AS methods: definition, importance, tandem techniques, applications, trends. 13th week: Challenges and solutions in sample preparation for atomic spectrometry.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
Learning outcomes
The purpose of the course is to introduce students to the theoretical foundations of modern atomic spectrometry methods used in inorganic elemental analysis. It covers key techniques such as optical atomic absorption and emission spectrometry, inorganic mass spectrometry, and subvalence electron spectrometry. The course places a strong emphasis on current trends, technological advancements, and analytical characteristics. Students will explore the practical applications of these methods across various fields, focusing on real-world sample analysis and strategies to address specific analytical challenges.
By the end of the course, students will understand the principles and procedures of the most common methods of atomic spectral analysis and will be able to choose an appropriate method for solving specific analytical tasks.
Prerequisites
Basic knowledge of instrumental analysis at the level of a bachelor's degree in technical chemistry.

Assessment methods and criteria
Written examination

Theoretical knowledge in the area of selected methods of atomic spectrometry will be tested by a written exam.
Recommended literature
  • Encyclopedia of Analytical Chemistry. .
  • Černohorský T., Jandera P. Atomová spektroskopie.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester