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Lecturer(s)
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Česlová Lenka, doc. Ing. Ph.D.
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Course content
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Basic principle of chromatography, classification of chromatographic techniques. Thermodynamic and kinetic aspects of chromatography, evaluation of data, efficiency of separation. Quality of chromatographic separation, quantitative and qualitative analysis, accuracy of the analysis. Thin layer chromatography and paper chromatography. Gas chromatography - principle, instrumentation (carrier gas, injectors, detection, columns), applications. High-performance liquid chromatography and ultra-performance liquid chromatography - principle and instrumentation, preparation and chosing stationary phase, new type of stationarz phases and supports. Separation in normal and reversed phase systems. Hydrophilic interaction liquid chromatography. Separation of ionic compounds (influence of pH). Gradient elution. Size exclusion chromatography. Bioafinity chromatography. Supercritical fluid chromatography. Chiral separations. Twodimensional chromatography - GCxGC, LCxLC, LCxCE, CExCE. Types and applications. Classification of electromigration separation techniques. Capillary electrophoresis - principle, factors affecting migration, techniques, applications. Isotachophoresis, micellar electrokinetic chromatography, electrochromatography, applications of electromigration techniques. Coupling of separation techniques and mass spectrometry - GC/MS, HPLC/MS, CE/MS. Ionization techniques, analyzators - new trends in instrumentation. Tandem mass spectrometry. Application.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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The main objective of the course is to extend basic knowledge of students in the field of chromatography and electromigration analytical separation techniques. The aim is to obtain such experience, so that listeners can assess the suitability of various analytical techniques and methods and critically assess achieved results. The topics of the course are theoretical background, instrumentation and the use of various chromatographic and electromigration techniques for analyzing substances depending on the size of molecules, polarity, or ion character.
Graduate of the course is able to assess the suitability of different analytical procedures and methods and critically evaluate the results achieved, is aware of the theoretical background, instrumentation and the use of various chromatographic and electromigration techniques for analysis of substances depending on the size of molecules, polarity, or ion character.
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Prerequisites
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Instrumental analytical chemistry, physical chemistry and organic chemistry.
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Assessment methods and criteria
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Oral examination
Test and oral examination
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Recommended literature
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Churáček J. a kol. Analytická separace látek. Praha, 1990.
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Churáček J. a kol. Nové trendy v teorii a instrumentaci vybraných analytických metod. Praha, 1993.
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Krejčí M., Pajurek J., Komers R. a kol. Výpočty a veličiny v sorpční kolonové chromatografii. Praha, 1990.
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Smolková E., Feltl L. Analýza látek v plynném stavu. Praha, 1991.
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