| Course title | Trace Analysis |
|---|---|
| Course code | KALCH/C854 |
| Organizational form of instruction | Lecture |
| Level of course | Master |
| Year of study | 2 |
| Semester | Winter |
| Number of ECTS credits | 3 |
| Language of instruction | Czech |
| Status of course | Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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Introduction to the trace analysis. Definition of terms, areas of application, types of samples, problems of trace analysis. Sensitivity, limits of detection and quantification, statistical evaluation of results and methods. Good laboratory practice. Systematic control of methods and results; standards, reference materials and their preparation. Purity of chemicals. Types of samples and their sampling, storage and preprocessing of samples before analysis. Extraction and purification techniques. Extraction by liquid. Extraction distillation, codistillation. Solid phase extraction, experimental setup, sorbents. Supercritical fluid extraction. Derivatization. Experimental setup, reasons for derivatization in HPLC and GC, examples. Instrumental methods in trace analysis: chromatography, spectral methods, electrochemical methods, radiochemical methods. Hyphenation of separation and spectral methods. Introduction, reasons for hyphenation and information quality of results. Classical tests, immunologic, enzymatic and biologic methods, application of optical and other sensors. Analysis of technical samples, water, air and gases, food, agricultural samples, soils, physiological samples, clinical and forensic analysis. Local analysis of selected classes of compounds (N-nitrosoamines, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polychlorinated dibenzodioxims and dibenzofurans).
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| Learning activities and teaching methods |
| Monologic (reading, lecture, briefing) |
| Learning outcomes |
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Trace analysis course introduces students to theoretical and practical aspects of the analysis of samples with trace concentrations and shows interdisciplinarity of the trace analysis. Students will learn to use knowledges aquired in other more specifically oriented courses to solve problems in trace analysis.
The students receive an overview of the preparation of the samples using instrumental methods of analysis and statistical evaluation of the results achieved in the determination of trace concentrations of analytes. |
| Prerequisites |
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Knowledges of analytical, physical (and organic) chemistry on the bachelor degree level.
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| Assessment methods and criteria |
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Oral examination, Written examination
Knowledges of subjected matter being lectured. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2013) | Category: Special and interdisciplinary fields | 1 | Recommended year of study:1, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2016) | Category: Special and interdisciplinary fields | 2 | Recommended year of study:2, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2015) | Category: Special and interdisciplinary fields | 2 | Recommended year of study:2, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2013) | Category: Special and interdisciplinary fields | 2 | Recommended year of study:2, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2015) | Category: Special and interdisciplinary fields | 1 | Recommended year of study:1, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Analysis of Biological Materials (2016) | Category: Special and interdisciplinary fields | 1 | Recommended year of study:1, Recommended semester: Winter |