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Lecturer(s)
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Foret František, Ing. DSc.
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Klepárník Karel, Ing. CSc.
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Course content
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Basic concepts of miniaturization procedures - photolithography, etching, replication procedures. Material selection. Prerequisites for practical use and basic ideas about the most important applications. Principles of DNA, protein and oligosaccharide analysis using microfluidic elements. The issue of intellectual property protection
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Learning activities and teaching methods
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Dialogic (discussion, interview, brainstorming), Methods of individual activities
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Learning outcomes
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The student will gain an overview of the basic techniques and principles of miniaturized analytical methods, including their biological applications.
The graduate has an overview in the theory and application of miniaturized separation and spectral techniques, including their coupling, as well as in the application area of DNA, protein and oligosaccharide analysis using microfluidic devices.
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Prerequisites
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Knowledge at the level of analytical chemistry courses - separation and spectral techniques, in the range of master degree studies.
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Assessment methods and criteria
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Oral examination
Oral examination
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Recommended literature
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Lazar I.M., Grym J., Foret F. Microfabricated devices: a new sample introduction approach to mass spectrometry. 2006, ISSN 0277-7037.
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Madou, Marc J. Fundamentals of microfabrication : the science of miniaturization. Boca Raton: CRC Press, 2002. ISBN 0-8493-0826-7.
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Novotny J., Foret F. Fluid manipulation on the micro-scale: Basics of fluid behavior in microfluidics. 2017, ISSN 1615-9306.
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