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Lecturer(s)
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Česlová Lenka, prof. Ing. Ph.D.
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Course content
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High-performance liquid chromatography, ultra high-performance liquid chromatography, gas chromatography, electromigration separation techniques - principle and application for the analysis of natural substances. Isolation of target substances from the natural matrix, sample preparation, optimization of separation and evaluation of results. Discussion of the connection of separation techniques with different spectral techniques - advantages, disadvantages, limitations, etc.
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Learning activities and teaching methods
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unspecified, Work with text (with textbook, with book)
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Learning outcomes
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The course focuses on extending the basic knowledge of chromatographic and electromigration analytical separation techniques and their coupling with mass spectrometry in the analysis of substances contained in natural matrix. The aim is to obtain such theoretical knowledge that the students will be able to assess the suitability of application of individual analytical procedures and methods for the analysis of complex mixture of substances depending on the molecular size, polarity or ionic character of the target analytes.
Graduate of the course is able to assess the suitability of different analytical procedures and methods and critically evaluate the results achieved, is aware of the theoretical background, instrumentation and the use of various chromatographic and electromigration techniques for analysis of different natural samples.
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Prerequisites
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completed master degree
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Assessment methods and criteria
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Oral examination, Self project defence
oral and written exam
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Recommended literature
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Churáček J. a kol. Analytická separace látek. Praha, 1990.
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Kromidas, Stavros. Quantification in LC and GC : a practical guide to good chromatographic data. Weinheim: Wiley-VCH, 2009. ISBN 978-3-527-32301-2.
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Li, S. F. Y. Capillary electrophoresis : principles, practice and applications. Amsterdam: Elsevier, 1992. ISBN 0-444-89433-0.
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Niessen, W. M. A. Liquid chromatography - mass spectrometry. Boca Raton: Taylor & Francis, 2006. ISBN 0-8247-4082-3.
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Poole, C. F.. Gas chromatography. Amsterdam: Elsevier, 2012. ISBN 978-0-12-385540-4.
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Sparkman, O. David. Gas chromatography and mass spectrometry : a practical guide. Burlington, Mass.: Academic Press, 2011. ISBN 978-0-12-373628-4.
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