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Lecturer(s)
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Žáková Pavla, Mgr. Ph.D.
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Kanďár Roman, prof. Mgr. Ph.D.
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Course content
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Sample preparation for analysis, the possibility of influencing the analysis, preanalytical effects Method establishment, analytical parameters, comparison of methods Automation in clinical biochemistry laboratories Laboratory information system, connection with automated analyzers and hospital information system Photometric methods, molecular spectroscopy, fluorimetry, nephelometry, turbidimetry, atomic absorption spectrometry, inductively coupled plasma emission spectrometry, examples of applications in biochemical laboratories Mass spectrometry in biochemical laboratories, application examples Immunochemical methods in laboratories of clinical biochemistry Chromatographic techniques used in biochemical laboratories, application examples Electrophoretic techniques used in biochemical laboratories, application examples Electrochemical methods used in biochemical laboratories, application examples Toxicological examination in clinical biochemistry laboratories Therapeutic drug monitoring Methods for determining the degree of oxidative stress
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming)
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Learning outcomes
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The aim of the course is to acquaint students with the issue of instrumentation in biochemical laboratories.
After completing the course the student is able to orientate in the problems of instrumentation in biochemical laboratories.
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Prerequisites
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not specified
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Assessment methods and criteria
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Oral examination
The exam is oral. The basic form of the exam is a discussion on selected topics. The degree of acquired knowledge is examined.
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Recommended literature
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Jaroslav Racek. Klinická biochemie. Praha: Galén, 2006. ISBN 80-7262-324-9.
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ZIMA, T. Laboratorní diagnostika. Praha: Galén, 2013.
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