University of Pardubice
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for academic year 2025/2026
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Course: General Biochemistry I

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Course title General Biochemistry I
Course code KBBV/C274
Organizational form of instruction Lecture + Seminary
Level of course Bachelor
Year of study 2
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Štěpánková Šárka, Mgr. Ph.D.
Course content
Biochemistry - history and presence. Characteristics of living organisms. Chemical composition of living matter. Hierarchy of structures. Biomolecules, biopolymers. Principles of matter and energy transformation. Bioenergetics. Cell - evolution, hierarchy and function. Biomembranes - structure, properties, function. Membrane transport. Viruses. Aminoacids - overview, acid-base balance, isoelectric point, optical activity, typical properties, reactivity. Methods for determination of aminoacids. Peptides and proteins - peptide bond, nomenclature, function, structure. Denaturation. Complex proteins (immunoglobulins, hemoproteins). Methods for determination of peptides and proteins. Saccharides - basic properties, function, importance. Mono-, oligo- and polysaccharides. Glycosidic bond. Reactions of saccharides. Derivatives of saccharides. Structural function of poly saccharides. Homo- and heteropolysaccharides, proteoglycans and glycoproteins. Poly- and oligosaccharides in cell communication, epitops. Practical aspects (dextran, hyaluronate). Methods for determination of saccharides. Nucleic acids - structure, building blocks. Bases and their tautomeric forms, nucleosides, nucleotides, minority bases. DNA, RNA and its types. Structure and function of nucleic acids. Complementarity of bases. Genetic code. Genome, mitochondrial genome. Regulation of gene expression. Replication, transcription, posttranscriptional modifications, translation, posttranslational modifications. Mutation. Genetically related diseases. Nucleoproteins. Methods for determination of nucleic acids. Lipids - structure, importance, division. Fatty acids, acylglycerols, waxes. Steroids. Complex lipids - phospholipids, glycolipids, prostaglandins. Lipoproteins. Methods for determination of lipids. Endocrine system - hierarchy, hormones of individual endocrine glands. Methods for determination of hormones. Vitamins - importance, division. Vitamin deficiency disorders. Methods for determination of vitamins. Enzymes - division into classes, nimenclature, structure. Cofactors. Enzyme kinetics. Effectors. Buffer systems in living organisms. Bicarbonate buffer. Hemoglobin - transport of oxygen.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
Learning outcomes
According the subject, students will be acquainted with a basic knowledge concerning biochemistry of living subjects and they will gain knowledge about the structure and importance of biomolecules and biopolymers.
Student will gain knowledge about structure and function of biopolymers, cell structure, hormonal regulation, enzyme kinetics and mechanism of homeostasis.
Prerequisites
unspecified

Assessment methods and criteria
Oral examination, Written examination

Presence at seminar lessons. Examination written and possibly oral. written test (success rate 50% at least) oral exam (2 exam questions)
Recommended literature
  • Garrett RH. Biochemistry, 4. vydání. Brooks/Cole: Cengage Learning, 2010.
  • Korecká, Lucie. Obecná biochemie : pro speciální chemicko-biologické obory. Pardubice: Univerzita Pardubice, 2012. ISBN 978-80-7395-470-3.
  • LEDVINA, M.; STOKLASOVÁ, A.; CERMAN, J. Biochemie pro studující medicíny I+II. Praha: Karolinum, 2011.
  • Štěpánková Š. Obecná biochemie. Pardubice, 2020. ISBN 978-80-7560-342-5.
  • ŠTERN P. a KOL. Obecná a klinická biochemie pro bakalářské obory studia, Nakladatelství Karolinum 2005.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
University of Pardubice, date of update: 16.03.2026 23:51. Data created for academic year 2025/2026