|
Lecturer(s)
|
-
Pejchalová Marcela, doc. Ing. Ph.D.
-
Heidingsfeld Olga, RNDr. CSc.
|
|
Course content
|
Physical and chemical properties of nucleic acids (NA), their functions. Spectrofotometric determination of NA. Isolation of NA. Electrophoresis of nucleic acids. Replication, transcription, translation of NA. Polymerase chain reakctions. Gene, genotype, allels. Bacterial genome. Viral genome. Cloning of DNA. GMO.
|
|
Learning activities and teaching methods
|
|
Monologic (reading, lecture, briefing)
|
|
Learning outcomes
|
The objective is to acquaint students with structure, properties and functions of nucleic acids. A special attention is marked to genome of bacteria and viruses.Basic information about isolation of nucleic acids, real-time PCR, sequenation and hybridisation techniques, respectivelly. Disscused is also cloning of DNA,preparation and application og genetically modified organisms.
Students adopted basic information about methods of isolation of nucleic acids and molecular-biological methods (PCR, real-time PCR, RFLP, sequenation, hybrydization techniques), which are currently used in detection of inherited diseases.
|
|
Prerequisites
|
C501 Biology C607 Biochemistry
|
|
Assessment methods and criteria
|
Oral examination, Written examination
written or oral examination
|
|
Recommended literature
|
-
Dorian J. Pritchard, Bruce R. Korf. Základy lékařské genetiky. Praha, 2007. ISBN 978-80-7262-449-2.
-
Jan Kapras a kol. Kontrolní otázky Biologie a lékařská genetika. Praha, 1999. ISBN 80-7254-081-5.
-
Mazura I. Speciální metody molekulární biologie. Karolinum, Praha., 2001. ISBN 802460258.
|