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Lecturer(s)
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Morávek Ondřej, Mgr.
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Jankovičová Barbora, Mgr. Ph.D.
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Course content
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1.-2. week: Isolation of DNA from different types of biological materials. 3.-4. week: DNA spectrophotometry, calculation of isolated DNA concentration. 5. week: Horizontal agarose gel electrophoresis. 6.-7. week: week: PCR, amplicon electrophoresis. 8.-9. week: week: Length polymorphism of restriction fragments 10. week: Electrophoresis of DNA fragments in polyacrylamide gel 11.-12. week: Interpretation of molecular detection of pathogens. 13. week: Search training in the Human Genome Mutation Database
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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Students will acquire basic knowledge of nucleic acid processing.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Home assignment evaluation
Checking logs
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Recommended literature
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KOČÁREK, E. Molekulární biologie v medicíně. 1. vyd. Brno: NCO NZO, 2007.
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Snustad P.D., Simmons M.J. Genetika.. Brno, 2009.
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Šmarda J, Doškař J, Pantůček R, Růžičková V, Koptíková J. Metody molekulární biologie. Brno: Masarykova univerzita, 2005.
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