|
Lecturer(s)
|
-
Čapek Jan, Mgr. Ph.D.
-
Roušar Tomáš, prof. RNDr. Ph.D.
|
|
Course content
|
The course aims to describe to students the work in the cell laboratory and the evaluation of basic toxicity determinations, which are performed in the study of cytotoxicity. Teaching will take place in three blocks, where the first block will focus on counting cells (suspension, adherent), their seeding, cultivation; the second will focus on processing and basic viability tests (proliferation), ie WST-1 test and TrypanBlueExclusion; the third will focus on the processing and evaluation of cytotoxicity after treatment with a model toxin depending on time and dose using a viability test and another cytotoxicity test (evaluation of oxidative stress or mitochondrial activity). Block 1 (week 1-4) - cell culture, basics of work in the cell laboratory Block 2 (week 5-8) - evaluation of cell viability Block 3 (weeks 9-13) - evaluation of cytotoxicity using routine cell tests
|
|
Learning activities and teaching methods
|
|
Laboratory work
|
|
Learning outcomes
|
The aim of the course is to describe at a basic level the work in the cell laboratory and the evaluation of basic determinations of toxicity, which are performed in the study of cytotoxicity. Teaching will take place in three blocks, where the first block will focus on counting cells (suspension, adherent), their seeding, cultivation; the second will focus on processing and basic viability tests (proliferation), ie WST-1 test and TrypanBlueExclusion; the third will focus on the processing and evaluation of cytotoxicity after treatment with a model toxin depending on time and dose using a viability test and another cytotoxicity test (evaluation of oxidative stress or mitochondrial activity).
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
Written examination
Participation in laboratories.
|
|
Recommended literature
|
-
Freshney R.I. Culture of animal cells (A manual of basic technique and specialized application). 2015.
-
1. Butler M. Cell culture and upstream processing. 2007.
|