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Lecturer(s)
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Roušar Tomáš, prof. RNDr. Ph.D.
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Čapek Jan, Mgr. Ph.D.
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Course content
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Cell cultre in vitro Cell laboratory equippment Primary cells, cell lines, cancer cell lines - their use in biomedicine Adherent and suspension cell lines Cryopreservation Proliferation of cells, cell counting, cell viability, flow cytometry, cell sorting Real time cell assessments, cytotoxicity, cell migration. Apoptosis/necrosis assessments Mitochondrial activity measurements Microscopy - fluorescence, bright field, phase contrast, intracellular cell probes Instrumentation Self work with cutting-edge scientific papers
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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The aim is to provide an overview on convenient and advanced methods for cell analysis. Students have an access to the information on equippment and standard processes in cell laboratory, incl. preservation, cell culture, and cell testing in vitro. Basal and advanced cellular methods are described, incl. cell differentiation protocols, proliferation, apoptosis, etc. The subject C479 is a prerequisity for following subjects: stem cells, molecular-biological methods, cell toxicology, etc.
The students are provided with the knowledge from advanced cell analysis methods, incl. cell proliferation, differentiation, and apoptosis.
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Prerequisites
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The subject C479 follows the knowledge from Cytology and Molecular biology and genetics.
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Assessment methods and criteria
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Written examination, Self project defence
Self work - presentation Written exam
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Recommended literature
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Alberts, Bruce . Essential cell biology. New York: Garland, 2010. ISBN 978-0-8153-4129-1.
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Alberts, Bruce. Základy buněčné biologie : úvod do molekulární biologie buňky. Ústí nad Labem: Espero, 2005. ISBN 80-902906-2-0.
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Freshney R.I. Culture of animal cells (A manual of basic technique and specialized application). 2015.
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Nečas, O. a kol. Obecná biologie pro lékařské fakulty. H&H, Jinočany 2000.
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1. Butler M. Cell culture and upstream processing. 2007.
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