| Course title | Laboratory of Microbiology |
|---|---|
| Course code | KBBV/C635 |
| Organizational form of instruction | Lesson |
| Level of course | Bachelor |
| Year of study | 3 |
| Semester | Winter |
| Number of ECTS credits | 2 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Safety and principles of work in microbiological laboratory, aseptic work, microbiological laboratory equipment. Methods of sterilization and disinfection. Basic terminology. Nutrient media (liquid and solid) - preparation and sterilization. Inoculation of microorganisms, practice of various inoculation techniques, isolation of pure culture, preservation and recovery of bacterial cultures. Cultivation of aerobic, anaerobic, microaerophilic microorganisms. Growth of microorganisms on solid and liquid nutrient media - determination of macroscopic characteristics. Physical, chemical and biological factors influencing the growth and reproduction of microorganisms - pH, temperature, UV radiation, osmotic pressure. Microscopic techniques (bright and dark field microscopy, fluorescence m., electron m.). Staining techniques - Gram staining. KOH test. Negative staining. Staining of spores and capsules. Acid-fast staining. Giemsa staining. Viruses - laboratory diagnostics. Cultivation of fungi important in the field of human microbiology - cultivation media, microscopic techniques. Biochemical tests based on carbohydrate transformation, transformation of nitrogen compounds. Other biochemical tests - production of hydrogen sulphide, production of catalase, oxidase, utilization of citrate. Commercial biochemical tests.
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| Learning activities and teaching methods |
| Laboratory work |
| Learning outcomes |
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Students are going to acquire the knowledge and skills to conduct various techniques used in laboratory of microbiology.
Students become acquainted with staining techniques, preparation of nutrient media and identification of microorganisms. Completion of the course is necessary for the further studies of special and clinical microbiology. |
| Prerequisites |
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KBBV/C604 - Optical microscopy techniques in health service
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| Assessment methods and criteria |
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Written examination
The knowledge is verified by written examination. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2015) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2013) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2016) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2014) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |