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Lecturer(s)
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Morávek Ondřej, Mgr.
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Štěpánková Šárka, Mgr. Ph.D.
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Course content
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Labour protection in biochemical lab, fire safety. Volume admeasurement, pipetting test, precision. Buffers, pH measurement. Aminoacids - determination of absorption maximum and molar absorption coefficient of chosen aminoacids, quantitative determination of aminoacids Proteins - determination of total protein using biuret reaction, precipitation of proteins by inorganic acids, organic solutions and inorganic salts.. Qualitative determination of saccharides. Quantitative determination of glucose. Lipids - determination of cholesterol in oils. Preparative chromatography of serum lipids and their quantification. Kinetics of enzymatic reaction, determination of Michaelis constant of urease. Determination of urea concentration in human serum. Determination of IC50 of butyrylcholinesterase inhibitors. Determination of vitamin A and C. Final test.
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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The aim is to improve practical competency of student for application measuring physical and chemical properties of substrates, enzymes, vitamins, hormones and nucleic acids.
Student will be able to work in laboratories of biochemistry including centrifugation, extraction, protein precipitation, analytical and preparative chromatography, HPLC, spectrophotometric measurements. Student can to statistically interpret obtained results.
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Prerequisites
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not specified
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Assessment methods and criteria
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Written examination
credit test
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Recommended literature
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návody IS STAG. .
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Štěpánková Š., Královcová P., Kanďár R. Laboratorní cvičení z obecné a klinické biochemie (druhé doplněné vydání). Pardubice: Univerzita Pardubice, 2018. ISBN 978-80-7560-174-2.
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Štěpánková, Žáková, Kanďár. Laboratorní cvičení z obecné a klinické biochemie. Pardubice. 2022.
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