1st week: Sample collection and preparation for microbiological analysis (methodology, standards). 2nd week: Analytical and experimental methods in microbiology. 3rd week: Microbial cell (construction of a prokaryotic and eukaryotic cell). Planktonic and biofilm forms of cells. 4th week: Microbial biofilm (formation process, composition, structure). Live vs. dead microbial cells. VBNC forms. 5th week: Experimental methods of biofilm detection - phenotypic methods (spectrophotometry), genotypic methods (PCR, FLU microscopy), use of intercalation dyes to determine cell viability. 6th week: Cultivation methods in microbiology (traditional design vs. commercial cultivation kits). 7th week: Special molecular-biological methods in microbiology (modification of PCR, FISH, PFGE, RFLP, etc.) for identifying microorganisms. 8th week: Biosensors and their use in microbiology. MALDI-TOF MS and the Biolog system for the identification of microorganisms. 9th week: Microorganism sensitivity testing - phenotypic methods (diffusion, dilution, and microdilution tests according to applicable standards), genotypic methods. Current trends in instrumentation and evaluation of test results. Resistance of microorganisms (forms, emergence, testing methods). 10th week: Staphylococcus aureus toxins and their detection (ELISA, RPLA, biosensors, PCR methods). Practical laboratory use, commercial assemblies. 11th week: Bacillus cereus and Clostridium botulinum toxins (HPLC/MS, ELISA, RPLA, PCR methods). Practical laboratory use, commercial assemblies. 12th week: Indirect methods in microbiology (ATP bioluminescence, spectrophotometry, turbidimetry, radiometry, etc.). 13th week: Modern and fast methods in microbiology.
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CLSI. Clinical and Laboratory Standards Institute. .
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EUCAST. European Committee on Antimicrobial Susceptibility Testing. .
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Králová, Blanka. Bioanalytické metody. Praha: Vysoká škola chemicko-technologická, 1995. ISBN 80-7080-234-0.
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VOTAVA M. Lékařská mikrobiologie obecná, Neptun Brno, 2001.
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Votava M. Mikrobiální biofilmy. Univerzita Palackého v Olomouci, 2011.
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