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Lecturer(s)
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Korecká Lucie, doc. RNDr. Ph.D.
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Course content
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Physical and chemical methods of immobilization of biomolecules on solid phase (basic division, principles) Types of solid phases for immobilization of biomolecules usable in immunoanalytical methods, biotechnological applications, drug-delivery systems Overview of functional groups of biomolecules (proteins, carbohydrates, NK) usable for immobilization Activating, conjugating and stabilizing agents Methods of preparation of labeled biomolecules for immunoassay, biotechnological applications, imaging methods (biotinylation, labeling with enzymes, fluorophores) Verification methods for determining the effectiveness of immobilisation and conjugation with a labeled ligand
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Learning activities and teaching methods
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Work with text (with textbook, with book), Methods of individual activities
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Learning outcomes
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The aim is to provide an overview of immobilization and conjugation techniques used to modify biologically active substances - antibodies, enzymes, affinity ligands. Overview of materials and types of carriers used for immobilization - nanomaterials, magnetic, non-magnetic. Ligands used for conjugation with biologically active substances, enzymes, fluorophores, nanomaterials.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination
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Recommended literature
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Cao, Linqiu. Carrier-bound immobilized enzymes : principles, applications and design. Weinheim: Wiley-VCH, 2005. ISBN 3-527-31232-3.
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Häfeli, U., Schütt, W., Teller, J., Zborowski, M. Scientific and Clinical Applications of Magnetic Carriers. 1997.
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Hermanson, Greg T. Bioconjugate techniques. Amsterdam: Elsevier, 2013. ISBN 978-0-12-382239-0.
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