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Lecturer(s)
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Kanďár Roman, prof. Mgr. Ph.D.
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Course content
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Definition of metabolomics. Targeted and non-targeted metabolite analysis. Metabolites and their profiling. Fingerprinting and footprinting of metabolites. Human metabolomics. Sample preparation for metabolic studies, dried spot technique of biological sample (dried blood spot, dried plasma spot, dried urine spot, etc.). The methods used in metabolomics (liquid and gas chromatography with mass detection, capillary electrophoresis with mass detection, tandem mass spectrometry, and nuclear magnetic resonance).
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
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Learning outcomes
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The subject introduces the student to knowledge in the field of metabolomics. It builds on the knowledge of biochemistry and analytical chemistry.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination
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Recommended literature
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Alessandra Sussulini. Metabolomics: From Fundamentals to Clinical Applications. Cham: Switzerland, 2017. ISBN 978-3-319-47655-1.
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John C. Lindon; Jeremy K. Nicholson; Elaine Holmes. The Handbook of Metabonomics and Metabolomics. Oxford: United Kingdom, 2007. ISBN 978-0-44-452841-4.
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Wolfram Weckwerth. Metabolomics ? Methods and Protocols. New Jersey: USA, 2007. ISBN 978-1588295613.
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