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Lecturer(s)
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Pejchalová Marcela, doc. Ing. Ph.D.
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Course content
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Chromogenic media. Immunochemical methods (ELISA, LFIA). Molecular biological methods (qualitative PCR, quantification using rel-time PCR). Genotyping methods (Gel electrophoresis in a pulsed field, Amplified Fragment Length Polymorphism, REP-PCR, ERIC-PCR). Sequencing methods (Multi Locus Sequence Typing, Whole Genome Sequencing). Identification of microorganisms using MALDI-TOF-MS analysis.
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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Detailed introduction of students to the latest methods of detection of food pathogens
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Prerequisites
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unspecified
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Assessment methods and criteria
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unspecified
Oral and written exam Preparation of a seminar paper on a given topic
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Recommended literature
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Doyle, Michael P. Food microbiology : fundamentals and frontiers. Washington: ASM Press, 2007. ISBN 978-1-55581-407-6.
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Motarjemi, Yasmine. Emerging foodborne pathogens. Cambridge: Woodhead, 2006. ISBN 1-85573-963-1.
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Robinson, R. K. (Richard Kenneth). Encyclopedia of food microbiology. San Diego: Academic Press, 2000. ISBN 0-12-227070-3.
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