Week 1: General principle of spectroscopic methods; introduction and development of NMR spectroscopy, use of NMR spectroscopy, spin and magnetic moment, nuclear spin, spin quantum number, spin and magnetic moment of atomic nuclei. Week 2: Atomic nucleus in a magnetic field, energy of nuclear quantum states, NMR signal intensity. NMR spectroscopy, nuclear spins and their surroundings, Larmor frequency, macroscopic magnetization, rf action. pulse, FID, Fourier transform. Week 3: Shielding and shielding constant, chemical shift, chemical shift scale, factors influencing chemical shift values. Week 4: Spin-spin interaction of nuclei with I = 1/2, interaction constant, heteronuclear spin-spin interaction, factors influencing the value of the interaction constant and its relation to the structure of molecules. Week 5: Examples of experimental NMR spectra and practicing the design of theoretical NMR spectra for simple organic and inorganic molecules with emphasis on nuclei with I = 1/2. Basics of working in software needed for processing 1D NMR spectra and processing of obtained experimental data. Week 6: Spin-spin interaction of nuclei with I> 1/2, and corresponding heteronuclear spin-spin interaction, differences from nuclei with nuclear spin I = 1/2. Natural representation of isotopes, satellites, hetero- and homonuclear decoupling. Exercises on corresponding concrete examples. Week 7: Classification of spin systems, higher order interactions, chemical vs. magnetic equivalence of spins. Week 8: Mechanisms of relaxation, and their consequences in measuring the NMR phenomenon. Dynamic processes in NMR spectroscopy. Week 9: Introduction to 2D NMR spectroscopy, use and basic division of 2D NMR spectra. Week 10: Basic methods 1H, 1H - COSY, 1H, 13C-HMBC and HMQC. Working with software for 2D NMR data processing. Week 11: Other available 2D NMR techniques and their use in describing the structure of organic and inorganic compounds. Week 12: Applications using 1D and 2D NMR spectroscopy for specific complex examples. Independent solution of experimental tasks from NMR spectroscopy, ie. complete assignment of NMR spectra of different samples in relation to their structure Week 13: Solid state NMR - CP / MAS NMR differences against solution.
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