1st week: Introduction to cell biology - cell compartmentation. Cell membranes - structure, function, transport of substances. 2nd week: Cell nucleus - structure, function, nucleic acids, chromosomes, DNA synthesis, gene, genetic code. 3rd week: Endoplasmic reticulum, Golgi complex, cytoskeleton. Cell cycle and its regulation. 4th week: Mitochondria - structure, function, ATP formation, mitochondrial DNA. 5th week: Basic types of eukaryotic cells. Cell surface structures - adhesive molecules, intercellular junctions, protein structures. 6th week: Stem cells - classification, properties. Stem cell plasticity. Stem cell niches. 7th week: Cell death - classification (apoptosis, necrosis, autophagy, senescence). Malignant cell transformation. 8th week: Operation of a cell culture laboratory - material and technical security, hygiene, equipment. 9th week: Cell isolation, culture protocols - passaging of adherent and suspension cell lines. Culture conditions. In vitro cell differentiation. 10th week: Overview and properties of biomaterials - biocompatibility, biodegradability, immunogenicity, cytotoxicity. 11th week: Biomaterials for 2D and 3D cell line cultivation - natural vs. artificial scaffolds. 12th week: Decellularization and advanced methods of 3D cell culture cultivation. 13th week: Use of cell cultures in regenerative medicine and tissue engineering.
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Pokyny v elektronické podobě dostupné všem studentům v IS STAG.
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ALBERTS, B. a kol. Základy buněčné biologie (Úvod do molekulární biologie buňky). 2. vyd. Ústí nad Labem: Espero, 2005. ISBN 80-902906-2-0.
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Freshney R.I. Culture of Animal Cells (A Manual of Basic Technique and Specialized Applications). New Jersey, 2010.
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Mather J. P., Roberts P. E. Plenum press Introduction to Cell and Tissue Culture (Theory and Technique). New York and London, 1998. ISBN 0-306-45859-4.
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