| Course title | Laboratory of Clinical Biochemistry |
|---|---|
| Course code | KBBV/C632 |
| Organizational form of instruction | Lesson |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Winter |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Safety at work in laboratories of clinical biochemistry. Laboratory information system. Analytical performance of the method (linearity, precision, recovery, detection limit). Electrolytes and microelements, importance and possibilities of determination electrolytes and microelements important for clinical practice. Enzymes, importance and possibilities of determination. Enzyme kinetics. Determination of enzymes and isoenzymes. Proteins, importance and possibilities of determination of total protein and albumin. Electrophoresis. Determination of selected proteins of acute phase. Non-protein nitric compounds. The comparison determination of uric acid by enzymatic method with method HPLC. Determination of creatinine and urea. Investigation of glomerular filtration. Lipids. Importance and possibilities of determination of total plasma cholesterol, HDL-C, LDL-C, triglycerides, ApoA-I and ApoB, calculation the total cholesterol-HDL-C/HDL-C ratio. Blood pigments: importance and possibilities of determination. Determination of bilirubin, hemoglobin and its derivates including glycated hemoglobin. Spectrophotometric determination of MDA (malondialdehyde) as a marker of oxidative stress. Glucose: importance and possibilities of determination in human serum. Biochemical and toxicological investigation of urine. Detection of blood in stool. The excursion: Department of Clinical Biochemistry, Regional hospital of Pardubice.
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| Learning activities and teaching methods |
| Laboratory work |
| Learning outcomes |
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The aim is to prepare student for working in laboratories of clinical biochemistry.
Student will be able to work in laboratories of clinical biochemistry. |
| Prerequisites |
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KBBV/C628 Laboratory of Biochemistry; KALCH/C625 Analytical chemistry II Laboratory Course
KBBV/C628 ----- or ----- KBBV/C638 and KALCH/C373 ----- or ----- KALCH/C625 |
| Assessment methods and criteria |
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Oral examination, Written examination
Protocols from all laboratory practices. Final test. Oral examination after failing test. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Chemical Technology | Study plan (Version): Laboratory Assistant (2015) | Category: Health service | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2015) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Laboratory Assistant (2013) | Category: Health service | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2013) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Laboratory Assistant (2016) | Category: Health service | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Laboratory Assistant (2014) | Category: Health service | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2016) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |
| Faculty: Faculty of Chemical Technology | Study plan (Version): Clinical Biology and Chemistry (2014) | Category: Special and interdisciplinary fields | 3 | Recommended year of study:3, Recommended semester: Winter |