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Lecturer(s)
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Stříbrná Lucie, Mgr. Ph.D.
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Pejchalová Marcela, doc. Ing. Ph.D.
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Course content
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1. Instruction to the safety at work. 2. Preparation and evaluation of prenatal diagnostic cytogenetic preparations (water, tissueof chorion). Evaluation findings, differential diagnosis, correlation with ultrasonic. 3. The preparation and evaluation of the postnatal diagnostic preparations (peripheral blood). Evaluation findings (use of molecular diagnostic differential). 4. Onkogenetics - cultivation and processing, bone marrow cells. Correlation of in the diagnosis, prognosis. Onkogenetics and monitoring treatment. 5. Cultivation and processing peripheral blood lymphocytes, obtained for chromosomal aberrations (ZCA). Cytogenetic methods in use. Chromosome instability syndromes. ¨ 6. Dyeing techniques and chromosome banding pattern, use in clinical practice. 7. Molecular cytogenetic methods: fluorescent in situ hybridization (FISH), arrayCGH, the latest trends. The use of differential diagnostic methods for the selection of the appropriate methodology. 8. The treatment of clinical genetics laboratory to clinical outcome and an anamnesis. 9. In cytogenetic laboratory quality control audits the internal quality control, quality control program. Model legislation and ethics, the situation in practice. 10. The isolation of NK theoretical introduction and practical role. 11. Electrophoresis NK theoretical introduction and separation of DNA. 12. Nobility.Examples of population genetics. H - W. law. 13. Model test. 14. Controling protocols, writing credits in the index of students.
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Learning activities and teaching methods
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Laboratory work
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Learning outcomes
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The main aim of this subject is to comprehend basic terms in genetics, inform about structure and function of nucleic acids and proteins. The main attention is paid to human genetics but also to genetic of bacteria and viruses. The others subjects are mutations and their types, sexual and asexual reproduction, cytogenetic and the most common genetic diseases in human population.
Students govern basic cytodiagnostic imaging techniques and basic methods used in molecular biology laboratories
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Prerequisites
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unspecified
KBBV/C501
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Assessment methods and criteria
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unspecified
Credit is granted after all laboratory tasks, the development of appropriate protocols.
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Recommended literature
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Nussbaum R. L., McInnes R. R. , Willard H. F. Klinická genetika (6. vydání). Praha, 2004.
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Pritchar D.J., Korf B.R. Základy lékařské genetiky. Galén, 2007.
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Snustad P.D., Simmons M.J. Genetika.. Brno, 2009.
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1. Kapras Jan a kol. Kontrolní otázky. Biologie a lékařská genetika.. praha, 1999. ISBN 80-7254-081-5.
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1. Otová Berta a kol. Lékařská biologie a genetika I.díl.. Praha, 2010. ISBN 978-80-246-1594-3.
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2. Kohoutová M. a kol. 2. Kohoutová M. a kol. Lékařská biologie a genetika II.díl. Praha, 2012. ISBN 978-80-246-1873-9.
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